结球甘蓝AFLP、SSR、SRAP标记高密度遗传图谱构建  

Construction of a Genetic Linkage Map in Cabbage Based on AFLP,SSR and SRAP Markers

作　　者：朱洪运[1,2] 颉建明[1] 郁继华[1] 简元才[2] 康俊根[2] 

机构地区：[1]甘肃农业大学农学院,甘肃兰州730070 [2]北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物生物学与种质创制重点实验室,北京100097

出　　处：《华北农学报》2013年第3期82-87,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31171958);“十二五”国家科技支撑计划项目(2012BAD02B01);“863”项目(2012AA100105);北京市科委重大项目(D111100001311002;D121100003412001)

摘　　要：以不同生态型甘蓝抗枯萎病高代自交系R4-P1和感枯萎病自交系R2-P2组配得到的142个F2单株作为构图群体,利用AFLP、SSR、SRAP这3种分子标记技术对该群体进行遗传连锁分析,构建了一张含有9个连锁群、240个标记位点的高密度甘蓝分子遗传连锁图谱(LOD≥4),其包含162个AFLP标记、52个SSR标记和26个SRAP标记,标记间平均图距3.6 cM,覆盖864.4 cM。该图谱涉及标记种类多,是目前发表的覆盖基因组较全面的结球甘蓝亚种内遗传连锁图谱,可供参考的SSR标记数量多,将为后期甘蓝枯萎病抗性基因的定位及克隆搭建良好平台,同时为结球甘蓝重要农艺性状的QTL定位及分子标记辅助选育新品种奠定基础。A F2 population consisting of 142 individual plants derived from a cross between fusarium wilt resistant cabbage self line R4-P1 and fusarium wilt susceptible line R2-P2 combining with AFLP,SSR and SRAP markers were applied to construct a high density genetic linkage map of Brassica oleracea.In total,240 markers including 162 AFLP markers,52 SSR markers and 26 SRAP markers were integrated into 9 groups（LOD≥4）.It covered 864.4 cM,with the average interval of 3.6 cM.It is a relatively high dense map involving more kinds of markers especially more reference SSR markers.It is also one of the most comprehensive intrasific genetic linkage map of covering whole genome of cabbage.It will build a good platform for mapping and cloning of the fusarium wilt resistant gene in cabbage,and furthermore,lay the foundation for mapping important agronomic traits for marker assistant breeding.

关 键 词：结球甘蓝 分子标记 遗传连锁图谱 

分 类 号：S635.03[农业科学—蔬菜学]