玉米自交系AS-9化学诱变后代SSR遗传变异分析  

Genetic Variation Analysis in Maize Mutants from AS-9 Inbread Line Based on SSR Marker

作　　者：李红英[1] 卢存福[1] 兰小中[2] 杨凤娇[1] 金德善[1] 乔佩[1] 卢骁[1] 玉猛[1] 陈玉珍[1] 

机构地区：[1]北京林业大学生物科学与技术学院,林木育种国家工程实验室,北京100083 [2]西藏农牧学院,西藏林芝860000

出　　处：《华北农学报》2013年第3期92-101,共10页Acta Agriculturae Boreali-Sinica

基　　金：国家科技支撑计划项目(2011BAI13B06);北京市自然科学基金(6112016);国家自然科学基金(31270737)

摘　　要：为分析系列化学诱变对玉米自交系AS-9诱变后代的影响,以玉米自交系AS-9及其化学诱变自交第1代M1和第4代M4为材料,进行发芽试验,同时利用分布在10条染色体上的68对SSR引物进行遗传变异分析。结果表明:诱变M1和M4种子的发芽率、芽长和根数普遍高于对照;能在2代玉米试材中扩增出多态性条带的引物有54对,占引物总数的79.41%;M1诱变系与基础材料对照的遗传相似系数平均值为0.364 7,遗传相似系数中心化后的数据在-0.12处,可将材料分为2个类群,基础材料(ⅠCK)为一类,诱变系为一类;M4诱变系与基础材料对照的遗传相似系数平均值为0.434 6,遗传相似系数中心化后的数据在-0.09处,可将材料分为基础自交系(ⅣCK)和诱变系2个类群;M1和M4主成分分析与聚类结果基本一致。说明基础材料AS-9与诱变系材料间存在真实的遗传差异,系列化学诱变已使玉米自交系AS-9产生了广泛的遗传变异。Analysis of genetic diversity in maize germplasm is of great importance to conserving and utilizing these valuable chemical mutagenic resources.In this research,the genetic differences between mutants（M1M4） and the corresponding orginal material（AS-9） was studied based on SSR markers with selected 54 polymorphism SSR primers.The results showed that the seed germination rate,bud length and root number of M1 M4 were higher than AS-9 in most cases.In two generations maize materials,54 polymorphism primers can be amplified,accounting for 79.41% of the total.Relatived toⅠCK,the average value of genetic similarity coefficient of M1 was 0.364 7.With the certered genetic similarity coefficient of-0.12 by UPGMA,the maize inbred line materials were divided into two groups：the orginal material（ⅠCK） and mutants.Relatived to ⅣCK,the average value of genetic similarity coefficient of M4 was 0.434 6.With the cemtered genetic similarity coefficient of-0.09,the maize materials were classified into two groups：ⅣCK and mutants.Principal component analysis and clustering results were similar.The results showed there were distinct variation between orginal material AS-9 and induced material,indicating chemical mutagen had produced genetic variations in maize inbred lines on large scale.

关 键 词：玉米 自交系 化学诱变 SSR标记 遗传变异 

分 类 号：S335.3[农业科学—作物遗传育种]