水分胁迫对油茶容器苗生理生化特性的影响  

Effects of water stress on physiological and biochemical characteristics of Camellia oleifera seedlings

作　　者：张诚诚[1] 曹志华[1] 胡娟娟[1] 文佳[1] 段文军[1] 李春生[2] 束庆龙[1] 

机构地区：[1]安徽农业大学林学与园林学院,合肥230036 [2]安徽省林业高科技开发中心,合肥230031

出　　处：《安徽农业大学学报》2013年第4期623-626,共4页Journal of Anhui Agricultural University

基　　金：安徽省农转基金项目(12040302005);安徽省农业科技成果转化项目(1104a0303065)共同资助

摘　　要：以一年生油茶容器苗为试验材料,采用称重法控制基质含水量,设置CK,T1,T2,T3,T4和T5 6个水分处理组,研究水分胁迫下容器苗的生理生化特征差异。结果表明:(1)CK组的叶片含水量和自由水/束缚水值都为最大,分别为68.5%和26.4,而其他4组随着基质含水量减少其叶片含水量和自由水/束缚水值逐渐减小。(2)相对电导率、过氧化物酶活性和丙二醛含量都随着基质含水量的减少而呈先减后增的趋势,其最低值拐点出现在T1组,分别为37%、11.1 U·min-1·g-1和0.3μg·g-1。(3)游离脯氨酸和可溶性糖的含量随着基质含水量的减少而增加,由CK组的231.4μm·g-1和40.4 mg·g-1增加到T4组的681.4μg·g-1和48.9 mg·g-1。综上所述,81%～90%的基质含水量最适合油茶容器苗木生长;当基质含水量超过90%时,叶片气孔开度减小,不利于苗木光合作用和呼吸作用等生理过程;当基质含水量下降至81%以下时,苗木开始受到干旱胁迫。在今后的容器育苗生产中,应保持基质合适的水分含量,以维持其正常生长。One-year-old seedlings of Fengyang No.1were chosen as experimental materials,which were grown in six substrates with different water contents by weighting method（CK,T1,T2,T3,T4 and T5）,and the difference of physiological and biochemical characteristics of C.oleifera seedlings were determined and analyzed.The results were as follows.（1） The leaf water content and the ratio of free water to bound water in CK group were the largest,and the values were 68.5% and 26.4,respectively.However,those in the other four treatments declined with the decrease of substrate water content.（2） The relative conductivity,POD activity and MDA content decreased first,and then increased with the decline of substrate water content.In addition,those values in T1 group were the smallest,being 37%,11.1 U·min^-1·g^-1and 0.3 μg.g^-1,respectively.（3） The content of free proline content and soluble sugar rose with the decline of substrate water,which varied from 231.4 μg.g^-1and 681.4 μm.g^-1in CK group to 40.4 mg.g^-1 and 48.9 mg.g^-1in T4 group,respectively.（4） When the substrate water content was above 90%,the reducing of leaf stomatic aperture was not beneficial for photosynthesis and respiration of the seedling.While the substrate water content dropped to less than 81%,the seedlings would suffer drought stress.Therefore,substrate water content of 81%-90% was the most suitable for seedlings growth.The substrate water content should be controlled in the appropriate range to keep seedlings growing normally in C.oleifera production.

关 键 词：油茶容器苗 水分胁迫 游离脯氨酸 可溶性糖 丙二醛 含水量 相对电导率 POD 

分 类 号：S794.4[农业科学—林木遗传育种]