镉锌处理麦苗对中华稻蝗(Oxya chinensis)体内锌、镉水平及生化指标的影响  

Effects of Cadmium and Zinc in Wheat Seedlings on Heavy Metal Accumulation and Biochemical Indices of Oxya chinensis

作　　者：朴君[1,2] 张育平[3] 杨慧敏[1,2] 吴海花[2] 张建珍[2] 马恩波[2] 李丽君[4] 郭亚平[1] 

机构地区：[1]山西大学生命科学学院,太原030006 [2]山西大学应用生物学研究所,太原030006 [3]太原师范学院,太原030012 [4]山西省农业科学院农业环境与资源研究所,太原030006

出　　处：《农业环境科学学报》2013年第7期1328-1333,共6页Journal of Agro-Environment Science

基　　金：国家自然科学基金项目(31071980);山西省自然科学基金(2011011033-1);高等学校博士点基金(20111401110006)

摘　　要：为了研究Cd、Zn污染对中华稻蝗生理生化的影响,选用不同浓度Cd、Zn溶液(Cd0+Zn0,Cd20+Zn0,Cd20+Zn50,Cd20+Zn100,Cd20+Zn200)mg·L-1培养麦苗饲喂中华稻蝗(Oxya chinensis)进行慢性染毒,测定麦苗和虫体(4龄若虫)中Cd与Zn蓄积量、虫体中丙二醛(MDA)含量、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GPx)活性和总抗氧化能力(T-Aoc)。研究结果显示,在Cd浓度恒定的情况下,随Zn处理浓度的增大,麦苗和虫体中Zn含量显著上升,(Cd20+Zn200)mg·L-1处理组麦苗和虫体Zn含量分别为对照组的2.03和1.63倍;Cd含量呈先升高后降低趋势,在(Cd20+Zn100)mg·L-1处理组达到最高值,分别为10.40、4.35μg·g-1,(Cd20+Zn200)mg·L-1处理组下降到7.36、3.35μg·g-1。在Cd和Zn联合作用下,随着Zn浓度的增加,中华稻蝗体内的MDA含量升高。SOD表现出先升高、后降低的趋势。与对照组相比,Cd单一处理组GPx活性增加,随着Zn的加入,其GPx活性降低。T-Aoc在(Cd20+Zn100)mg·L-1处理组活性最高。研究结果表明,低浓度Zn的加入可以增加中华稻蝗对Cd的富集,Cd和Zn的联合作用能够引起中华稻蝗脂质过氧化损伤。In order to study the interaction effects of cadimun（Cd） and zinc（Zn）, Oxya chinensis were fed with the wheat seedlings cultured in solutions with different concentrations of Cd^2＋ and Zn^2＋. Accumulation of Cd and Zn in the wheat seedlings and the grasshoppers were measured. The content of malondialdehyde（MDA）, superoxide dismutase（SOD） activity, glutathione peroxidase（GPx） activity and total antioxidant capacity（T-Aoc） in the grasshoppers were analyzed. The results showed that Zn contents increased significantly in the seedlings and grasshoppers when Zn concentration was increased and the Cd concentration was constant in solutions. When treated with the mixture of 20 mg·L^-1 Cd^2＋ and 200 mg·L^-1 Zn^2＋, Zn contents in the seedlings and grasshoppers were 2.03 and 1.63 times higher than that in the control, respectively. Cd contents showed a tendency of first increase and then decline, and reached the highest when treated with mixture of 20 mg·L^-1 Cd^2＋ and 100 mg·L^-1 Zn^2＋. The contents of MDA increased in the grasshoppers with Zn concentration increasing under the interaction of Zn and Cd. SOD activity firstly increased then declined. Compared with the control, GPx activity increased when treated with 20 mg·L^-1 Cd^2＋ alone and decreased when treated with the mixture of Cd and Zn. T-Aoc content reached the highest under the treatment of 20 mg·L^-1 Cd^2＋ and 100 mg·L^-1 Zn^2＋. These results indicated that low concentrations of Zn could increase the accumulation of Cd in O. chinensis. The interaction of Zn and Cd could cause the damage of lipid peroxidation.

关 键 词：中华稻蝗 镉 锌 相互作用 生化指标 

分 类 号：X503.223[环境科学与工程—环境工程]