1株鸡传染性支气管炎病毒变异株的分子特征和抗原性分析  

Molecular and antigenic characterization of a novel infectious bronchitis virus isolate

作　　者：胡北侠[1,2] 文心田[2] 杨少华[1] 曹三杰[2] 许传田[1] 张琳[1] 黄艳艳[1] 张秀美[1] 

机构地区：[1]山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室,山东济南250100 [2]四川农业大学动物医学系,四川雅安625014

出　　处：《中国兽医学报》2013年第7期991-994,共4页Chinese Journal of Veterinary Science

基　　金：山东省自然科学基金资助项目(ZR2009DQ015);现代农业产业技术体系建设专项资金项目(CARS-42-Z12)

摘　　要：从山东省发病商品鸡群分离鉴定了1株鸡传染性支气管炎病毒,命名为CK/CH/SD09/005。S1基因序列分析发现,该株和同期分离的16个IBV流行株与疫苗株H120和491同源性分别为65.2%和66.0%。BLAST发现,CK/CH/SD09/005株仅与GenBank中3个广东分离株同源性较高(98.6%～98.8%),并形成独立的进化群。分别以H120和491为参考株,除了广泛的点突变,CK/CH/SD09/005S1基因在多个位点发生了插入和缺失,CK/CH/SD09/005N基因仅发生了点突变,没有基因插入和缺失;M基因除了发生点突变之外,分别在16～18和7～18位发生了碱基插入。鸡胚中和试验结果显示,CK/CH/SD09/005与H120和491均有交叉中和性,抗原相关性分别为0.18和0.09,应属于Mass和793/B之外新的血清型。结果表明,IBV S1基因可同时通过点突变、插入和缺失产生变异株,增加了免疫预防的难度。An infectious bronchitis virus（IBV） strain named CK/CH/SD09/005 was identified from commercial broilers of Shandong province in 2009. The S1 ,N and M genes of CK/CH/SD09/ 005 were sequenced and phylogenetically analyzed with IBV strains published in GenBank. The similarity of the nucleotide sequences of S1 genes between CK/CH/SD09/005 and sixteen Shan- dong IBV isolates was 54.3%-60.1% ,whereas CK/CH/SD09/005 and three Guangdong IBV iso- lates in GenBank were clustered into the same cluster, with $1 genes sequences identities of 98.6%-98.8%. With H120 and 491 as the reference strain,substitutions,insertions and deletions were found in many positions of CK/CH/SD09/005 S1 gene. As for M gene, besides substitu- tions,insertions were located in 16-18 bp and 7-18 bp of M gene 5r part. There is no insertion and deletion in N gene of CK/CH/SD09/005 except mutations. Although reciprocal neutralization was found between CK/CH/SD09/005 and H120, CK/CH/SD09/005 and 491, CK/CH/SD09/005 was neither Massachusetts nor 793/B serotype,as antigentic relatedness value of which was 0.18 and 0.09 respectively. The results showed that point mutation,insertion and deletion have contributed to the emergence of the variant IBV strain CK/CH/SD09/005.

关 键 词：传染性支气管炎病毒 变异株 分子特征 抗原性 

分 类 号：S852.657[农业科学—基础兽医学]