鹅源新城疫病毒的分离鉴定及其致病性  

Isolation,identification and pathogenicity of Newcastle disease virus from goose

作　　者：谷松至[1] 王新[1] 杜冬菊[1] 蒋运博[1] 胡桂学[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《中国兽医学报》2013年第7期995-998,共4页Chinese Journal of Veterinary Science

基　　金：吉林省科技厅项目(20040206-2-2);吉林省现代农业产业技术体系建设专项资金资助项目(201231)

摘　　要：从吉林省某发病鹅场分离到1株病毒,经血凝、血凝抑制试验和RT-PCR鉴定证实,该毒株为新城疫病毒,命名为MHK-1株。毒力测定结果表明,该病毒的鸡胚平均死亡时间(MDT)为43h,1日龄雏鸡脑内接种致病指数(ICPI)为1.63,鸡胚半数致死量(ELD50)为10-8.3/0.2mL,表明该毒株为新城疫病毒强毒株。经致病性试验表明,该病毒对鹅有很强的致病性。分子病毒学分析表明,MHK-1株F基因裂解位点的氨基酸序列为112 R-R-Q-K-R-F117,符合强毒株氨基酸序列特点。A strain virus isolated from a goose farm in Jilin province was identified as Newcastle disease virus by HA, HI and RT-PCR. The virus was named MHK-1 strain. The MDT and ICPI, ELD50 {or MHK-1 strain were 43 h,1.63 and 10-83/0.2 mL,respectively. The results indicated that the MHK-1 isolate was virulent strain of Newcastle disease virus. The pathogenic experiment revealed that the virus is very strong pathogenic to goose. The sequence analysis of F gene of the isolate showed that the sequence of the cleavage site of the fusion protein of MHK-1 isolate was 112 R-R-Q-K-R-F117 ,which is consistent with that of the published virulent NDV strains.

关 键 词：鹅源新城疫病毒 分离 鉴定 致病性 

分 类 号：S852.657[农业科学—基础兽医学]