ADF-IL-2基因重组卡介苗的构建及免疫保护效果  

Construction and immunoprotection of recombinant BCG with ADF-IL-2 gene

作　　者：侯洪烈[1,2] 张许科[3] 李运娜[2] 孙进忠 李建华[2] 宫鹏涛[2] 李赫[2] 杨举[2] 张西臣[2] 

机构地区：[1]辽东学院,辽宁丹东118001 [2]吉林大学动物医学学院,吉林长春130062 [3]国家兽用药品工程技术研究中心普莱柯生物工程股份有限公司,河南洛阳471003

出　　处：《中国兽医学报》2013年第7期1016-1020,共5页Chinese Journal of Veterinary Science

基　　金：丹东市科学技术计划项目支持(08107);国家“863”计划资助项目(2011AA10A215);国家自然科学基金资助项目(31072123)

摘　　要：构建柔嫩艾美尔球虫ADF基因和鸡IL-2基因的重组卡介苗,并研究其免疫保护性。将柔嫩艾美尔球虫的ADF基因和鸡的IL-2基因串联后连接到至穿梭表达载体pMV261和整合表达载体pMV361中,电转化卡介苗,制备重组卡介苗pMV261-ADF-IL-2和pMV361-ADF-IL-2,将构建的基因重组卡介苗分别以不同免疫途径接种雏鸡,研究其对柔嫩艾美耳球虫的攻毒的免疫保护效果。结果显示,成功构建重组rBCG pMV261-ADF-IL-2和pMV361-ADF-IL-2,且rBCG滴鼻免疫组的免疫效果较好,其抗球虫指数分别为176.08和172.89。结果表明,构建的基因重组卡介苗pMV261-ADF-IL-2和pMV361-ADF-IL-2对柔嫩艾美尔球虫卵囊的攻击具有一定的保护作用。The rBCG vaccine expressed Eimeria tenella ADF and chicken IL-2 （chlL-2） was con- structed and its efficacy against E. tenella challenge was observed. ADF gene of E. tenella and chIL-2 gene were cloned into pMV261 and pMV361 vector,respectively, the recombinant p lasmids were transformed into BSG by electroporation and producd rBCG carrying ADF-IL-2 gene. Chick- ens were immunized with rBCG pMV261- ADF-IL-2 and pMV361- ADF-IL-2 via different im- mune route and analyzed its immunoprotection against E. tenella. The result show the rt3CG vac- cines were successfully constructed and the ri3CG immuned with intranasal route showed the bet- ter efficacy against E. tenella than the other routes,their ACI were 176.08 and 172.89,respective- ly. The rBCG could provide an immunoprotection to chicken challenged E. tenella oocysts.

关 键 词：柔嫩艾美耳球虫 ADF IL-2 基因重组卡介苗 免疫保护 

分 类 号：S852.723[农业科学—基础兽医学]