脂多糖对奶山羊肝脏脂代谢关键基因表达的影响  

Effects of LPS on the expression of lipid metabolism key genes in liver of dairy goats

作　　者：李和平[1] 王月影[1] 刘凤娟[1] 祁画丽 王林枫[1] 汪新建[1] 张君[1] 杨国宇[1] 

机构地区：[1]河南农业大学农业部动物生化与营养重点开放实验室,河南郑州450002 [2]郑州牧业高等专科学校,河南郑州450008

出　　处：《中国兽医学报》2013年第7期1055-1058,1063,共5页Chinese Journal of Veterinary Science

基　　金：国家重点基础研究发展计划资助项目(2011CB100802)

摘　　要：12只初产萨能奶山羊被随机分为对照组和脂多糖(LPS)组,每组6只。试验结束后,采集肝脏组织,利用荧光定量PCR的方法检测肝脏TLR4信号通路关键分子、脂肪合成和转运关键基因的表达。结果显示,LPS能上调TLR4、MyD88、IRAK4和NF-κB基因的表达,下调LXRα、CD36、VLDLR、APOB、ACC和SCD的表达,对LXRβ和PPAR的表达影响不大。结果表明,LPS诱导肝损伤的同时能下调肝脏脂代谢基因的表达,进而降低肝脏合成与转运脂肪的能力。To clarify the effect of lipopolysaccharides （LPS）on the expression of lipid metabolism key genes in liver of dairy goats, providing theorical basis for metabolism and re-distribution of milk fat precursor in liver. Twelve primiparity Saanen dairy goats were randomly divided into con- trol group and LPS group of six each. At the end of experiment,liver tissue was obtained. The ex- pression of key genes for TLR4 signal pathway, lipid synthesize and transport was detected by fluorescence quantitative PCR methods. The results displayed that LPS could up-regulate the ex- pression of TLR4, MyD88, IRAK4 and NF-kB genes, down-regulate the expression of LXRα, CD36,VLDLR, APOB, ACC and SCD genes, no effect on the expression of LXRl3and PPAR genes. It suggested that LPS induced liver injury,and could up-regulate the expression of lipid metabolism genes in liver, and then decreased the ability of lipid synthesize and transport in liver.

关 键 词：脂多糖 奶山羊 肝脏 脂代谢关键基因 荧光定量PCR 

分 类 号：Q78[生物学—分子生物学] S852.23[农业科学—基础兽医学]