不同种类异位酸添加水平对奶牛瘤胃体外发酵的影响  

Effect of different level of three isoacids on rumen fermentation of dariy cow in vitro

作　　者：沈冰蕾[1] 苗树君[1] 曲永利[1] 邵广[2] 

机构地区：[1]黑龙江八一农垦大学动物科技学院黑龙江大庆163319 [2]黑龙江省八五一一农场,黑龙江密山158300

出　　处：《中国兽医学报》2013年第7期1086-1090,共5页Chinese Journal of Veterinary Science

基　　金：农垦总局项目(HNK11A-08-01-02);国家科技部项目(2012BAD12B05);国家“十二五”乳业专项子课题(2012BADO4A09)

摘　　要：选用3头装有永久性瘤胃瘘管的奶牛,采用单因素对照试验设计和体外培养法研究不同种类异位酸的单独添加水平对奶牛瘤胃发酵的影响,并利用多项指标综合指数对培养液pH值、氨态氮浓度、菌体蛋白含量和残留有机物的含量进行综合评定,筛选3种异位酸单独使用的适宜添加量。结果表明,异丁酸0.2%DM和0.4%DM、异戊酸为0.15%DM和0.3%DM、2-甲基丁酸为0.1%DM和0.2%DM添加方式为最佳。Three dairy cows fitted with permanent rumen fistula were chosen and single-factor con- trast design was applied in sole adding of three kinds of isoacids under the culture condition in vitro. Each level of isoacids would be done three times at 0,6,12 and 24 h, respectively. The study focus on the effects of internal environment of rumen, assessing culture fluid pH, the concentra- tion of ammonia nitrogen, the contents of bacterial crude protein and organic dry matter by MFAEI. Finally the appropriate adding dose of three kinds of isoacids was as follow, isobutyric acid 0.2%DM and 0.4%DM,isovaleric acid 0. 15%DM and 0. S%DM and 2- methyl nail acid 0.1% DDM and 0.2%DM.

关 键 词：异位酸 体外培养 瘤胃发酵 奶牛 

分 类 号：S856[农业科学—临床兽医学]