复方中药“禽康散”对鸭瘟病毒感染鸭胚及成纤维细胞的保护效应  

Protection of the Chinese herbal medicine "Qinkangsan" against duck plague virus in duck embryo and duck embryo fibroblasts

作　　者：任志华[1] 邓惠丹[1] 邓俊良[1] 刘小雪[1] 廖轶[1] 左之才[1] 王娅[1] 彭西[1] 崔恒敏[1] 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室环境公害与动物疾病四川省高校重点实验室,四川雅安625014

出　　处：《中国兽医学报》2013年第7期1095-1098,1118,共5页Chinese Journal of Veterinary Science

基　　金：“长江学者和创新团队发展计划”创新团队项目(IRT0848);四川省教育厅自然科学重点项目(2006A009);四川省科技厅应用基础重点项目(2008JY0098)

摘　　要：将不同浓度复方中药"禽康散"和鸭瘟病毒(Duck plague virus,DPV)分别以不同方式接种到鸭胚尿囊膜后观察其对DPV感染的鸭胚保护率;用MTT法测定"禽康散"对鸭胚成纤维细胞(DEF)的安全质量浓度和增殖的影响,及安全质量浓度范围内的"禽康散"对DPV感染鸭胚成纤维细胞(DEF)能力的影响。结果显示,"禽康散"在10日龄鸭胚内对DPV具有显著的杀灭和阻断感染的作用。"禽康散"对DEF的最大安全质量浓度为2-4 g/mL,在2-4～2-8 g/mL质量浓度范围内对DEF有显著的增殖和抑制DPV感染DEF的能力,且与加药方式和药物质量浓度有一定关系。结果表明,一定质量浓度的"禽康散"对DEF具有增殖作用,并对DPV在鸭胚内和细胞水平上均有较好的杀灭和拮抗作用。Duck embryo was treated with different dose in three ways （pre-, post-, and mixed-ad- ministration） to evaluate the effect of the Chinese herbal medicine Qinkangsan against DPV,test Qinkangsan safety concentrations for the growth of duck embryo fibroblast （DEF）. The effects of Qinkangsan on cellular infectivity of DPV were assayed by MTT method. The results indicated that Qinkangsan could inhibit the multiplication of DPV in duck embryo. The maximal safety con- centrations of Qinkangsan was 2-4 g/mL. The concentrations at 2 4-2-8 g/mL could promote the cell growth significantly and inhibit DPV to infect DEF significantly,which were related to the administration mode and drug concentrations.

关 键 词：复方中药“禽康散” 鸭瘟病毒 鸭胚 鸭胚成纤维细胞 

分 类 号：S853[农业科学—临床兽医学]