荧光定量RT-PCR检测性成熟前与性成熟山羊卵巢α/β-ER mRNA表达的比较  

Comparative study of the mRNA expression of α/β-estrogen receptors in prepubertal and pubertal goats with fluorescence quantitative RT-PCR assays

作　　者：王洪进[1] 李宏梅[1] 胡洪杰 刘海港[1] 窦文文[1] 井维芳[1] 郭慧君[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]山东省畜牧总站,山东济南250022

出　　处：《中国兽医学报》2013年第7期1113-1118,共6页Chinese Journal of Veterinary Science

基　　金：山东省中青年科学家奖励基金资助项目(2008BS07005);山东省博士后创新项目专项奖金资助项目(200803026)

摘　　要：为分析性成熟前与性成熟波尔山羊和沂蒙黑山羊卵巢内α、β雌激素受体(α/β-ER)表达的差异规律,根据已发表波尔山羊α/β-ER基因序列,设计不同引物,对PCR反应条件进行优化,建立稳定检测2种山羊卵巢雌激素受体mRNA含量的荧光定量RT-PCR方法;并对性成熟前后2种山羊卵巢组织中α/β-ER mRNA进行了检测比较。结果显示,性成熟前2种山羊卵巢α-ER和β-ER mRNA表达含量较性成熟山羊低;而且这2种山羊无论性成熟前还是性成熟后,α雌激素受体表达均显著低于β雌激素受体。To compared the mRNA expression of α/β-estrogen receptors（ERα.ERβ） in ovaries ob- tained trom Boer and Yimeng black goats in prepuberty and puberty, 6 couples of α/β-ER gene primers were designed and synthesized; the conditions of reaction such as annealing temperature and time, extending temperature and time, cycle times were optimized with gradient PCR or PCR assays;the mRNA expression of ERα, ERβ in ovary follioles were detected with above-optioned fluorescence quantitative RT-PCT assays. The results indicate that the mRNA expressions of ERα/ERβ in prepubertal ovaries of Boer goats and Yimeng black goats are less than those in pu- bertal ones, and the mRNA expression of ERa in ovaries of both goats, whether in prepuberty or in puberty, are less that of ER;. These results will be helpful to clarify the mechanism on different developmental competence of goats follicle oocytes in prepuberty and in puberty.

关 键 词：山羊 α、β雌激素受体 性成熟前 荧光定量RT—PCR卵巢卵泡 

分 类 号：Q78[生物学—分子生物学] S813[农业科学—畜牧学]