催情助孕液对小鼠雌、孕激素水平及其受体mRNA表达的影响  

Effect of Cuiqing zhuyunye on levels of estrogen and progesterone and their receptors mRNA expression in mouse

作　　者：张世栋[1] 王旭荣[1] 杨峰[1] 李宏胜[1] 潘虎[1] 王东升[1] 严作廷[1] 

机构地区：[1]中国农业科学院,兰州畜牧与兽药研究所,中国农业科学院临床兽医学研究中心,农业部兽用药物创制重点实验室,甘肃省新药工程重点实验室,甘肃兰州730050

出　　处：《中国兽医学报》2013年第7期1127-1131,共5页Chinese Journal of Veterinary Science

基　　金：国家“十二五”科技支撑计划重点课题资助项目(2012BAD12B03);兰州市科技计划项目(2010-1-219)

摘　　要：分别以0.1、0.2、0.4mL/d剂量的催情助孕液给21日龄小鼠灌服7d后,检测体质量、脏器指数、血清雌激素和孕激素水平、子宫组织中雌激素和孕激素受体基因mRNA的表达情况,并与腹腔注射雌二醇4d的小鼠进行比较。结果显示与对照组和雌二醇注射组比较,0.2mL的催情助孕液能显著促进小鼠子宫发育,并增强雌激素水平及其受体基因表达,而对孕激素水平及其受体基因表达无显著影响。结果表明,催情助孕液对动物生殖发育与促进发情具有较好的效果。To investigate the pharmacological mechanism of Cuiqing zhuyunye, and provide the the- oretical basis for clinical use. The mics born for 21 d were gavaged in stomach with 0.1,0.2 and 0.4 mL/d drugs for 7 d respectively,then weight,viscera index,serum estrogen and progesterone levels,mRNA expression of estrogen receptor （ER） and progesterone receptor （PR） in uterus were detected,and compared to the group with 5 mg/kg（per day） estradiol intraperitoneal injec- tion for 4 d. Compared with group of control negative and group of estradiol injection, estrogen level and ER mRNA expression were enhanced in 0.2 mL/d drug gavage, and uterine development was also boosted obviously. But there was no notable effect on progesterone and PR. There was better effect of Cuiqing zhuyunye on reproductive development and estrus stimulation of mice.

关 键 词：催情助孕液 雌激素 孕激素 雌激素受体-Α 孕激素受体 mRNA 

分 类 号：S814.8[农业科学—畜牧学]