愈肠宁胶囊中苦参提取物的生物碱类成分指纹图谱分析  

Fingerprint Analysis of Alkaloids from Extract of Sophora flavescens in Yuchangning Capsules

作　　者：向孙敏[1] 韩丽[1] 刘李梅[1] 张超[1] 王莹[1] 谢兴亮[2] 

机构地区：[1]成都中医药大学,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137 [2]成都医学院药学院,成都610083

出　　处：《中国实验方剂学杂志》2013年第14期13-16,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技重大专项“十二五”重大新药创制项目(2012ZX09102201-103)

摘　　要：目的:建立愈肠宁胶囊中苦参提取物生物碱类成分的HPLC指纹图谱的检测方法。方法:采用Kromasil NH2氨基柱(4.6 mm×250 mm,5μm),流动相乙腈-乙醇(8∶1)-3%磷酸水梯度洗脱,流速0.8 min.mL-1,检测波长220 nm,对10批苦参提取物进行HPLC指纹图谱检测,采用"中药色谱指纹图谱评价系统2004年版"进行评价。结果:苦参提取物采用氨基柱各峰分离效果好,确定了15个共有峰,并对其中4个色谱峰进行了指认,10批提取物的相似度均>0.9,提取物与药材指纹图谱间存在相关性。结论:该方法精密度、重复性、稳定性较好,为苦参提取物的质量控制提供了一种检测方法。Objective： To establish HPLC fingerprint detection for alkaloids from extract of Sophora flavescens in Yuchangning capsules.Method： Amino Kromasil NH2 column（4.6 mm × 250 mm,5 μm） was adopted,gradient eluted with mobile phase of acetonitrile-ethanol（8 ∶ 1）-3% phosphoric acid water,flow rate was 0.8 min.mL-1,detection wavelength was 220 nm.10 batches of extract of S.flavescens were detected by HPLC fingerprint,then evaluated with 2004 edition of ‘chromatographic fingerprint evaluation system of Chinese medicine’.Result： By taking Kromasil NH2 column,peaks separation of extract of S.flavescens was better,identified 15 common peaks and 4 of them were assigned.Similarity of these 10 batches extract was more than 0.9,correlation between fingerprints of extract and raw materials existed.Conclusion： This method had good precision,repeatability and stability,it could provide a detection method for quality control of extract of S.flavescens.

关 键 词：苦参 提取物 生物碱 指纹图谱 

分 类 号：R283.6[医药卫生—中药学]