铁皮石斛中总氨基酸的含量测定及提取工艺优选  

Optimization of Ultrasonic Extraction Technology and Content Determination of Total Amino Acids from Dendrobium officinale

作　　者：王培培[1] 鲁芹飞[1] 李婧[1] 黄松[1,2] 陈建南[1,2] 

机构地区：[1]广州中医药大学,广州510006 [2]东莞广州中医药大学中医药数理工程研究院,广东东莞523808

出　　处：《中国实验方剂学杂志》2013年第14期30-33,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十二五"科技支撑计划课题(2011BAI01B02);广东省科技厅中药现代化专项(K5080016);广东省科技计划项目(2008A030101009);广东省科技计划项目(2011A030100011);广东省教育部产学研结合项目(2010B090400474)

摘　　要：目的:优选铁皮石斛中总氨基酸提取工艺,并建立其含量测定方法。方法:以天冬氨酸为对照品,采用UV测定铁皮石斛中总氨基酸含量;在单因素试验基础上,采用L9(34)正交试验考察提取时间、提取次数及溶剂用量对铁皮石斛中总氨基酸提取工艺的影响。结果:最佳提取工艺为加20倍量0.1 mol.L-1HCl超声提取30 min,提取1次。结论:优选的提取工艺稳定可行,建立的含量测定方法简便、准确、重复性好。Objective： To optimize supercritical CO2 fluid extraction process parameters of volatile oil from Angelica sinensis.Method： With composite score of the content of ligustilide and yield of volatile oil as index,the content of ligustilide was determined by HPLC,L9（34） orthogonal test was used to investigate effect of extraction temperature,extraction pressure,extraction time on extraction efficiency.Result： Optimum process parameters were as follows： extraction pressure 30 MPa,extraction temperature 50 ℃,extraction time 2 h,flow rate of CO2 25 L.h-1,pressure of separation kettle I 8 MPa with temperature at 50 ℃,separation pot II pressure of the system tail pressure with temperature at 35 ℃.Conclusion： This optimized process was stable and feasible,which could be adopted to extract volatile oil from A.sinensis with high yield and good quality.

关 键 词：铁皮石斛 总氨基酸 紫外分光度法 含量测定 提取工艺 

分 类 号：R283.6[医药卫生—中药学]