冻融-酶解-膜分离复合技术纯化板蓝根多糖的工艺优选  

Optimization of Purification Technology for Polysaccharides from Isatis indigotica by Freezing and Thawing-Enzymolysis-Membrane Separation Composite Technology

作　　者：陈智[1,2] 陈凯[1] 田景振[1] 

机构地区：[1]山东中医药大学,济南250355 [2]济南市第二人民医院,济南250001

出　　处：《中国实验方剂学杂志》2013年第14期42-44,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省重大创新药物综合平台--中药创新药物平台项目(2009ZX09301-013)

摘　　要：目的:优选冻融-酶解-膜分离复合技术纯化板蓝根多糖的工艺。方法:以板蓝根多糖和剩余蛋白含量为指标,在单因素试验基础上,采用正交试验考察酶解温度、酶解时间、酶用量对板蓝根多糖纯化工艺的影响。结果:最佳纯化工艺为板蓝根残渣水提取液反复冻融3次,过滤,按3.5万U.g-1加入蛋白酶2,酶解温度35℃,酶解时间3 h,酶解后用截留相对分子质量30 kD中空纤维组件超滤。结论:优选的纯化工艺稳定可行,冻融-酶解-膜分离复合技术可用于板蓝根多糖的纯化。Objective： To optimize purification technology of polysaccharides from Isatis indigotica by freezing and thawing-enzymolysis-membrane separation composite technology.Method： With the content of polysaccharides from I.indigotica and remaining protein as indexes,based on single factor tests,orthogonal test was adopted to investigate effect of hydrolysis temperature,reaction time and enzyme dosage on purification technology.Result： Optimal purification process was as following： repeated freezing and thawing three times of water extract liquid of polysaccharides from I.indigotica residue,filtered,added protease 2 with dosage of 35 000 U.g-1,enzymatic temperature for 35 ℃,hydrolysis time of 3 h,then ultrafiltrated with hollow fiber components of interception molecular mass 30 kD.Conclusion： This optimized technology was stable and feasible,freezing and thawing-enzymolysis-membrane separation composite technology could be used to purify polysaccharides from I.indigotica.

关 键 词：板蓝根 多糖 纯化工艺 

分 类 号：R283.6[医药卫生—中药学]