核桃隔膜中总黄酮的提取工艺优选  

Optimization of Extraction Technology for Total Flavonoids from Diaphragm of Juglans regia

作　　者：张淑兰[1] 王国军[2] 唐辉[2] 刘艳芳[2] 

机构地区：[1]新疆生产建设兵团医院药剂科,乌鲁木齐830002 [2]新疆特种植物药资源教育部重点实验室,新疆石河子832002

出　　处：《中国实验方剂学杂志》2013年第14期50-52,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：新疆生产建设兵团科技项目(2011BA055)

摘　　要：目的:优选核桃隔膜中总黄酮的提取工艺。方法:以木犀草苷为定量指标,在单因素试验基础上,采用正交试验考察乙醇体积分数、料液比、原料粒径对总黄酮提取量的影响。结果:最佳提取工艺为过60目筛的原料加15倍量60%乙醇超声提取,总黄酮提取量达2.985 mg.g-1。结论:优选的总黄酮提取工艺稳定合理、重复性好,为核桃资源的充分利用提供实验依据。Objective： To optimize extraction technology of total flavonoids from diaphragm of Juglans regia.Method： With luteoloside as quantitative index,based on single factor tests,effect of ethanol concentration,solid-liquid ratio and mesh of raw material on yield of total flavonoids was investigated by orthogonal test.Result： Optimal extraction technology was as following： grinding degree of raw material 60 mesh,ultrasonic extracted with 15 times the amount of 60% ethanol,yield of total flavonoids was up to 2.985 mg.g-1.Conclusion： This optimized technology of total flavonoids was reasonable,reproducible and stable,it could provide basis for resources utilization of J.regia.

关 键 词：核桃隔膜 黄酮 正交试验 超声提取 高效液相色谱法 

分 类 号：R283.6[医药卫生—中药学]