HPLC测定散结镇痛胶囊中龙血素A,B含量  

Determination of Loureirin A,B in Sanjie Zhentong Capsules by HPLC

作　　者：鹿丽丽[1] 萧伟[2] 徐连明[2] 徐忠坤[2] 殷洪梅[2] 

机构地区：[1]南京中医药大学,南京210000 [2]江苏康缘药业股份有限公司,江苏连云港222001

出　　处：《中国实验方剂学杂志》2013年第14期105-108,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：科学技术部国家重大新药创制项目(2011ZX09201-201-20)

摘　　要：目的:建立散结镇痛胶囊中龙血素A、龙血素B的含量测定方法。方法:75%甲醇超声提取龙血素A、龙血素B,以Kromasil C18(4.6 mm×250 mm,5μm)为分析柱,以乙腈-1%冰醋酸为流动相进行梯度洗脱,流速1 mL.min-1,进样量10μL,检测波长278 nm,柱温30℃。结果:龙血素A在526.20～4.11 ng线性关系良好(r=0.999 9),平均回收率101.84%(RSD 0.77%,n=6);龙血素B在506.09～3.95 ng线性关系良好,r=0.999 9,平均回收率100.54%(RSD 1.46%,n=6);并具有较好的重复性和稳定性。结论:样品处理方法合理,方法学考察符合定量要求,结果准确,可用于散结镇痛胶囊中龙血素A、龙血素B的含量测定。Objective： To establish an HPLC method for content determination of loureirin A,B in Sanjie Zhentong capsules.Method： Extracted loureirin A,B with ultrasonic method in the use of 75% methanol,Kromasil C18 column（4.6 mm × 250 mm,5 μm） was used with a step gradient of acetonitrile-1% glacial acetic acid at a flow rate of 1.0 mL.min-1.The sample amount was 10 μL and the detection wavelength was set at 278 nm.The column temperature was maintained at 30 ℃.Result： Loureirin A and B had a good linear relationship during 526.20-4.11 ng（r = 0.999 9） and 506.09-3.95 ng（r = 0.999 9） respectively.The average recoveries were 101.84% with RSD 0.77%（n = 6）,100.54% with RSD 1.46%（n = 6）.The method also had good reproducibility and stability.Conclusion： The sample preparation is reasonable in the quantitative method,and the accurate results can be used for content determination of loureirin A and B of Sanjie Zhentong Capsules.

关 键 词：散结镇痛胶囊 龙血素A 龙血素B 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]