HPLC-ELSD测定复方甘草酸铵注射液中2种未衍生氨基酸的含量  

Direct Determination of 2 Underivatized Amino Acids in Compound Gancaosuanan Injection by HPLC-ELSD

作　　者：张建业 冯向东 

机构地区：[1]河南省南阳市食品药品检验所,河南南阳473000

出　　处：《中国实验方剂学杂志》2013年第14期161-163,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:用HPLC-ELSD法直接测定复方甘草酸铵注射液中2种未衍生氨基酸的含量。方法:使用Agilent-ODS C18(4.6 mm×250 mm,5μm)色谱柱,流动相A(乙腈)-B(5.0 mmol.L-1七氟丁酸的0.7%三氟醋酸溶液),梯度洗脱(0～5 min,0%A;5～10 min,15%A;10～25 min,35%A),ELSD漂移管温度80℃,氮气流量2.0 L.min-1,流速0.8 mL.min-1,柱温35℃,进样10μL。结果:甘氨酸进样量在4.858～29.145μg,盐酸半胱氨酸进样量在0.386～2.316μg时,其对数值与峰面积对数值线性关系良好,回收率分别为97.43%,96.99%。结论:测定方法简便,结果准确,可用于复方甘草酸铵注射液中2种氨基酸的测定。Objective： To determine 2 underivatized amino acids in Compound Gancaosuanan Injection with HPLC-ELSD.Method： The analysis was carried out on a Agilent-ODS C18（4.6 mm × 250 mm,5 μm） column.The mobile phase was acetonitrile（A）-5.0 mmol.L-1 heptafluorobutyric acid in 0.7% trifluoroacetic acid solution（B） in gradient elution mode（0-5 min,0% A;5-10 min,15% A;10-25 min,35% A）.Drift tube temperature was 80 ℃,gas flow rate was 2.0 L.min-1.The flow rate was 0.8 mL.min-1.Column temperature was kept at 35 ℃ and the inject volume was 10 μL.Result： The glycine and cysteine hydrochloride were linear in the range of 4.858-29.145 μg and 0.386-2.316 μg.The average recovery was 97.43%,96.99% respectively.Conclusion： The method is convenient and accurate,which can be used to determine 2 amino acids in Compound Gancaosuanan Injection.

关 键 词：复方甘草酸铵注射液 未衍生化 甘氨酸 盐酸半胱氨酸 高效液相色谱 蒸发光散射检测器 

分 类 号：R284.1[医药卫生—中药学]