艾纳香油对硫酸沙丁胺醇体外透皮吸收的影响  

Effect of Blumea balsamifera Oil on Percutaneous Absorption of Salbutamol Sulfate

作　　者：付万进[1,2] 王丹[1] 庞玉新[1] 王晖[2] 黄钊[2] 聂昊[2] 于福来[1] 张影波 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点开放实验室,海南儋州571737 [2]广东药学院中药学院,广州510006

出　　处：《中国实验方剂学杂志》2013年第14期174-177,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：海南省自然科学基金(312022);中央级公益性科研院所基本科研业务费专项资金(中国热带农业科学院热带作物品种资源研究所)项目(1630032012001);海南省中药现代化专项(2011ZY001)

摘　　要：目的:研究艾纳香油对硫酸沙丁胺醇透皮吸收的影响,为艾纳香油的应用提供理论依据。方法:采用改良Franz扩散池装置,以豚鼠背部皮肤为体外渗透屏障,采用紫外分光光度计方法测定接收液中硫酸沙丁胺醇的含量,考察0.5%,1%,2%艾纳香油,1%氮酮,以及1%艾纳香油与1%氮酮联合运用对硫酸沙丁胺醇透皮吸收的影响。结果:0.5%,1.0%艾纳香油和1.0%氮酮单独应用时对硫酸沙丁胺醇透皮吸收和贮库效应均具有显著促进作用(P<0.05)。但1.0%艾纳香油与1.0%氮酮合用时其促透效果较单独使用时降低,2.0%艾纳香油对硫酸沙丁胺醇透皮吸收无显著的促进作用。结论:低浓度的艾纳香油随着浓度升高对硫酸沙丁胺醇透皮吸收有增强作用,但高浓度艾纳香油对硫酸沙丁胺醇透皮吸收无显著促进作用。Objective： To study effects of Persicae Semen on apoptosis and protein expression of endothelial cell in cold stagnation and blood stasis syndrome（HS） and heat stagnation and blood stasis syndrome（RS） rats.Method： Eighty rats were randomly divided into 8 groups including normal control group of HS（HN）,model group of HS（HM）,Persicae Semen treatment group of HS（HT）,Chuanxiong Rhizoma control group of HS（HC）,normal control group of RS（RN）,model group of RS（RM）,Persicae Semen treatment group of RS（RT）,Salviae Miltiorrhizae Radix et Rhizoma control group of RS（RD）.To establish the two rat models by freezing and injection of carrageenan solution.Extracts of Persicae Semen,Chuanxiong Rhizoma and Salviae Miltiorrhizae Radix et Rhizoma were given to the corresponding group rats.Severn days later,Four hundred organs derived from the eighty rats were detected by histopathology analysis（HE,IHC and TUNEL）.The indexes included growth factor VEGF,apoptotic inhibitor Bcl-2,apoptotic induced protein p53 and apoptotic number of vascular endothelial cell（VEC）.Result： ①The corresponding blood stasis syndromes appeared in HM and RM rats,while improvements of the two syndromes appeared in HT and RT rats.② Decreased VEGF expression appeared just in RT rats（P 0.05）,while it did not change in HT rats.Increased Bcl-2 expression appeared just in RT rats（P 0.05）,while it did not change in HT rats.p53 expression did not change in both HT and RT rats.③ Apoptosis did not change in both HT and RT rats.Conclusion： Persicae Semen proved the blood circulation and suppressed apoptosis of endothelial cell in blood stasis rats,it might be caused Persicae Semen decreased VEGF and increased Bcl-2 expression of endothelial cells

关 键 词：艾纳香油 透皮吸收 渗透系数 增渗倍数 

分 类 号：R285.5[医药卫生—中药学]