桃仁对血液循环障碍大鼠内皮细胞凋亡及相关蛋白表达的影响  

Effect of Persicae Semen on Apoptosis and Protein Expression of Endothelial Cell Because in Rats with Blood Circulation Disorders

作　　者：以敏[1] 邓家刚[1] 郝二伟[2] 张锡流[3] 欧海玲[3] 崔静珠[3] 

机构地区：[1]广西医科大学药学院,南宁530021 [2]广西中医药大学中药药效重点实验室,南宁530001 [3]广西中医药大学第一附属医院病理科,南宁530023

出　　处：《中国实验方剂学杂志》2013年第14期178-182,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部973计划课题(2007CB512602);广西科学基金项目(桂科基09320005)

摘　　要：目的:了解桃仁对寒凝血瘀证(寒证)和瘀热互结证(热证)内皮细胞凋亡和相关蛋白表达的影响。方法:80只大鼠随机分为寒证对照组、模型组、桃仁(8 g.kg-1)组、川芎(8 g.kg-1)阳性对照组,以及热证对照组、模型组、桃仁(8 g.kg-1)组、丹参(14 g.kg-1)阳性对照组,每组10只。采用全身低温冷冻和注射角叉菜胶两种方法分别建立寒、热两证模型,造模同时各给药组,每天ig给药1次,连续给药7 d,取心、肺、肝、肾、脾,采用HE染色、免疫组化和原位末端转移酶标记技术(TUNEL)检测器官血管内皮细胞生长因子(VEGF)、B淋巴细胞瘤-2基因蛋白(Bcl-2)、肿瘤基因p53蛋白(p53)表达及细胞凋亡情况。结果:①两证模型大鼠均出现寒证和热证相应表征,用药大鼠相应表征均有改善;②桃仁用药后,血管内皮细胞增殖相关蛋白VEGF寒证无明显变化,热证表达明显减弱(P<0.05);凋亡蛋白Bcl-2寒证无明显变化,热证表达明显增强(P<0.05);凋亡蛋白p53寒热证均无明显变化;③桃仁用药后,两证血管内皮细胞凋亡数量均无明显变化。结论:桃仁改善寒、热两证大鼠血液循环障碍并抑制内皮细胞凋亡,其机制可能与减弱VEGF表达、增强Bcl-2表达以维持内皮细胞较高凋亡水平有关。Objective： The effects of the Blumea balsamifera oil on percutaneous absorption of albuterol sulfate were studied.Then,a theoretical basis for the application of B.balsamifera oil was proved.Method： The effects of six different penetration enhancer programs on albuterol sulfate transdermal absorption were determined,i e,0.5%,1.0%,2.0% B.balsamifera oil,1.0% azone,1.0% B.balsamifera oil jointed with 1.0% azone and black control.Concretely,the contents of albuterol sulfate in receiving fluid were determined by UV spectrophotometer using modified Franz diffusion cell device and the excised skin from guinea pig was taken as exoteric permeability barrier.Result： 0.5%,1.0% B.balsamifera oil and 1% azone individually salbutamol sulfate transdermal absorption and storage reservoir showed a significant role in promoting（P 0.05）.However,the effect of combination of 1.0% B.balsamifera oil and 1.0% azone on percutaneous penetration was lower than its using alone.Conclusion： With the increase of low concentration of B.balsamifera oil,the transdermal absorption of salbutamol sulfate enhanced.However,high concentration of B.balsamifera oil showed no significant difference.

关 键 词：桃仁 寒凝血瘀证 瘀热互结证 血液循环障碍 内皮细胞 凋亡 TUNEL法 

分 类 号：R285.5[医药卫生—中药学]