肾脾阳虚大鼠模型及桂附理中丸对大鼠海马衰老变化的影响  

Effects of Guifu Lizhong Wan on Hippocampus Aging in Mice Deficiency of Kidney and Spleen Model

作　　者：郭煜晖[1] 周安方[2] 陈涛[1] 胡卫[1] 梅志刚[1] 

机构地区：[1]三峡大学,湖北宜昌443002 [2]湖北中医药大学,武汉430065

出　　处：《中国实验方剂学杂志》2013年第14期182-185,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金青年项目(81102529)

摘　　要：目的:本实验首次采用im氢化可的松和ig大黄水煎液复合方法复制肾脾阳虚模型,观察桂附理中丸对肾脾阳虚大鼠大脑海马组织的影响以及温补脾肾方药的调节作用。方法:8周龄SD大鼠40只,雄性,随机分为正常对照组、模型组、维生素E组和桂附理中丸组4组。正常对照组大鼠每天上午8:00 im生理盐水5 mL.kg-1,同时ig生理盐水10 mL.kg-1,下午14:00 ig生理盐水10 mL.kg-1;其余组大鼠上午8:00 im氢化可的松25 mg.kg-1,同时ig大黄水煎液4 g.kg-1,下午14:00模型组ig生理盐水10mL.kg-1,维生素E组ig维生素E混悬液18×10-3g.kg-1,桂附理中丸组ig桂附理中丸混悬液1.62 g.kg-1,共30 d。电镜下观察海马组织超微结构改变;流式细胞仪检测细胞周期。结果:肾脾阳虚模型可使大脑海马CA1区细胞器及神经元出现衰老表现,细胞老化率明显升高(P<0.05),与模型组比较,维生素E组及桂附理中丸组大脑海马细胞器增多,脂褐素沉积减少,细胞老化率明显下降(P<0.05),桂附理中丸组大鼠海马CA1区细胞轮廓清晰,无细胞器及神经元细胞衰老征象。结论:实验大鼠海马组织的衰老变化是肾脾阳虚模型的明显病理改变,桂附理中丸具有调节海马神经细胞、恢复细胞周期的功效。Objective： To study the effects of Guifu Lizhong Wan on hippocampus aging in mice deficiency of kidney and spleen model.Method： Forty SD male mice were randomly divided into normal control group,model group,vitamin E group,Guifu Lizhong Wan group,10 each group.The normal control group was im treated with 5 mL.kg-1saline and ig with 10 mL.kg-1 at 8： 00 am,and 2： 00 pm,respectively.The other groups were injected hydrocortisone with 25 mg.kg-1and liquid of Rheum palmatum decoction were administered with 4 g.kg-1at 8： 00 am,the model group was administered saline with 10 mL.kg-1,vitamin E group was administered vitamin E with 18 × 10-3g.kg-1 and Guifu Lizhong Wan groups were given Guifu Lizhong Wan with 1.62 g.kg-1 at 2： 00 pm,once a day 30 days.Observation of ultrastructure of hippocampus changes;testing the brain hippocampus cells cycles by flow cytometry.Result： In Hippocampus the highest proportion of G1 phase（P 0.05） was found,compared with the model control group,the vitamin E and Guifu Lizhong Wan increased organization organelles with fewer neuron atrophy,and neurons synaptic gap widening,meanwhile the cell aging rate decreased obviously（P 0.05）.Conclusion： Guifu Lizhong Wan has an effect on hippocampus aging in mice deficiency of kidney and spleen model

关 键 词：肾脾阳虚 桂附理中丸 大脑海马 

分 类 号：R285.5[医药卫生—中药学]