比较地黄不同炮制品组方的四物汤对小鼠T淋巴细胞亚群的作用  

Comparision on the Effect of Different Production of Rehmanniae Radix in Siwu Prescription on T Lymphocyte Subsets in Mice

作　　者：李敏兰[1] 陈智钦[1] 张振凌[1] 

机构地区：[1]河南中医学院,郑州450046

出　　处：《中国实验方剂学杂志》2013年第14期215-218,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中医药行业专项[20110700701]

摘　　要：目的:比较地黄不同炮制品组成的四物汤免疫作用的区别,说明生地黄和熟地黄功效的异同。方法:昆明种雌性小鼠,第1,2,3天腹腔注射(ip)环磷酰胺(CTX)80 mg.kg-1建立免疫抑制模型,按体重随机分为模型对照组,阳性香菇多糖组(0.1 g.kg-1),鲜地黄-四物汤组(20.3 g.kg-1),生地黄-四物汤组(15.8 g.kg-1),熟地黄-四物汤组(15.8 g.kg-1),去当归-四物汤组(12.0 g.kg-1),每组10只,连续灌胃(ig)7 d。取脾脏和胸腺,计算脏器指数;采集淋巴细胞样品,用流式细胞仪进行检测,观察受试药物对模型动物CD3+,CD4+,CD8+淋巴细胞及CD4+/CD8+的表达水平。结果:熟地黄-四物汤组能显著提高免疫抑制小鼠的脾脏指数(7.26±0.85)mg.g-1和胸腺指数(0.75±0.12)mg.g-1(P<0.05),同时能够极显著提高CTX造成的免疫抑制小鼠CD3+(40.68±1.24)%,CD4+(48.08±2.14)%,CD8+(29.56±1.43)%淋巴细胞(P<0.01)及CD4+/CD8+(1.62±0.36),其他组无显著性差异。结论:熟地黄-四物汤能显著提高机体T细胞及其亚群间的平衡,鲜地黄-四物汤和生地黄-四物汤作用不显著,生、熟地黄组方四物汤其对免疫功能的作用差别较大。Objective： To compare the effects of different processed products of Rehmanniae Radix in Siwu prescription,and the difference between raw rehmannia and prepared rehmannia.Method： Kunming female mice were injected,on day 1,2,3 respectively celiac injection（ip） cyclophosphamide（CTX） 80 mg.kg-1 to establish immunosuppression model.After 7 days of administration,the spleen and thymus,acquisition lymphocyte sample were collected.Flow cytometry instrument was used to test CD3＋,CD4＋,CD8＋.Result： The prepared Rehmannia Radix of Siwu prescription could improve the immune suppression of spleen and thymus index（P 0.05）,and at the same time,improve CD3＋,CD4＋,CD8＋ lymphocytes（P 0.01） and CD4＋ / CD8＋ Conclusion： The prepared Rehmannia Radix of Siwu prescription can improve the T cell subsets.Rehmanniae Radix with different processed methods has an influence on the immune function.

关 键 词：地黄不同炮制品 环磷酰胺 免疫抑制 淋巴细胞亚群 

分 类 号：R285.5[医药卫生—中药学]