肝胆两益汤镇静催眠作用及其机制研究  

Sedative and Hypnotic Activities of Gandan Liangyi Tang via the Elevation Effects on Amino Acids Neurotranmitters in Mice

作　　者：张岗强[1,2] 何昕[3] 董宪喆[2] 刘屏[2] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中国人民解放军总医药临床药理研究室,北京100853 [3]蚌埠医学院,安徽蚌埠233030

出　　处：《中国实验方剂学杂志》2013年第14期226-229,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：军队"十二五"中医药推广研发项目(10ZYZ141)

摘　　要：目的:研究肝胆两益汤对小鼠的镇静催眠作用及其作用机制。方法:将KM小鼠随即分为正常组、安定组(1.3mg.kg-1)、百乐眠组(0.3 g.kg-1组)和肝胆两益汤组(5.2,2.6,1.3,0.65 g.kg-1),按照10 mL.kg-1ig给药,连续给药7 d,正常组ig等量蒸馏水。观察肝胆两益汤对小鼠ip戊巴比妥钠阈剂量;采用转棒法观察肝胆两益汤对小鼠运动协调性的影响,观察肝胆两益汤对小鼠自主活动的影响;高效液相色谱法检测肝胆两益汤对小鼠脑组织中氨基酸类神经递质[天门冬氨酸(Asp),谷氨酸(Glu),甘氨酸(Gly)和γ-氨基丁酸(GABA)]含量的影响。结果:肝胆两益汤2.6,1.3 g.kg-1组能显著缩短小鼠入睡潜伏期、显著延长小鼠睡眠持续时间和显著减低小鼠自主活动次数(P<0.05或P<0.01),而对小鼠运动协调性没有明显的影响。肝胆两益汤5.2 g.kg-1组能显著升高小鼠脑组织中GABA,Asp,Glu的含量(P<0.05或P<0.01),2.6 g.kg-1组能显著增加小鼠脑组织中GABA,Gly的含量(P<0.05或P<0.01),而1.3 g.kg-1组能显著升高小鼠脑组织中GABA的含量(P<0.01)。结论:肝胆两益汤有显著的镇静催眠作用,其作用机制可能与增加脑组织中抑制性氨基酸GABA和Gly的含量有关。Objective： To study the sedative and hypnotic effects of Gandan Liangyi Tang（GDLYT） and the potential mechanism in mice.Method： The KM mice were divided into seven groups： control group,diazepam group,Bailemian group,GDLYT 5.2,2.6,1.3,0.65 g.kg-1 dose groups,and mice were administrated once per day.The threshold dose of pentobarbital sodium was observed in mice.The locomotion activity was estimated,and the rotarod test was used to evaluate the effects of GDLYT.The contents of amino acids neurotranmitters including aspartic acid（Asp）,glutamic acid（Glu）,glycine（Gly）,γ-aminobutyric acid（GABA） in mice brain were determined by HPLC-FL.Result： The results showed that administration of GDLYT at 2.6,1.3 g.kg-1.d-1 significantly shorted the latency period and lengthened the duration of sleep（P 0.05 or P 0.01）.Besides,the locomotion activities were significantly reduced by GDLYT（1.3,2.6 g.kg-1.d-1）（P 0.05 or P 0.01） while the coordination of motion was no changed in mice after GDLYT treated.The results of HPLC indicated that GDLYT 5.2 g.kg-1group can significantly increase the GABA,Asp and the Glu content（P 0.05 or P 0.01）,and the 2.6 g.kg-1group could increase the GABA and Gly content significantly（P 0.05 or P 0.01）,meanwhile the 1.3 g.kg-1 group could significantly increase the content of GABA（P 0.01） in the mice brain tissues.Conclusion： Our results suggested that GDLYT exerts sedative and hypnotic activities,and the mechanisms may be correlated with the effects of increasing the contents of amino acids neurotranmitters GABA and Gly.

关 键 词：肝胆两益汤 镇静催眠 中枢神经递质 

分 类 号：R285.5[医药卫生—中药学]