马齿苋不同提取物对D-半乳糖致衰老小鼠的抗疲劳作用  

Anti-fatigue Action of Different Extracts from Portulaca oleracea in Senile Mice by D-Gal

作　　者：朴日龙[1] 吴红梅 张红英[1] 

机构地区：[1]延边大学医学院机能学实验中心,吉林延吉133002 [2]延边州结核病防治所,吉林延吉133000

出　　处：《中国实验方剂学杂志》2013年第14期240-243,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究马齿苋不同提取物对D-半乳糖致衰老小鼠抗疲劳能力的影响,并探讨其机制。方法:取小鼠随机分为正常组、模型组、马齿苋正丁醇提取物(0.5,0.25 g.kg-1)组、马齿苋乙醇提取物(1,0.5 g.kg-1)组。除正常组sc生理盐水外,其他各组均sc D-半乳糖(1 g.kg-1)42 d制备衰老小鼠模型。用疲劳仪测定小鼠的抗疲劳能力,并测定脑组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果:马齿苋正丁醇提取物(0.5 g.kg-1)和乙醇提取物(1 g.kg-1)组均可提高衰老小鼠的抗疲劳能力,马齿苋正丁醇提取物(0.5,0.25 g.kg-1)可降低脑组织MDA含量和提高SOD活性,马齿苋乙醇提取物(1 g.kg-1)可降低脑组织MDA含量。结论:马齿苋正丁醇提取物和乙醇提取物可提高衰老小鼠的抗疲劳能力,其机制可能与提高SOD活性降低MDA含量有关。马齿苋正丁醇提取物作用好于乙醇提取物。Objective： To study the anti-fatigue action of different extracts from Portulaca oleracea in senile mice by D-gal,and its mechanisms.Method： The mice were randomly divided into 4 groups： control group,model group,the n-butanol extracts from P.oleracea（0.5,0.25 g.kg-1） groups and the ethanol extracts from P.oleracea（1,0.5 g.kg-1） groups.The control group is given hypodermic injection of isotonic NaCl in the transverse part while the other groups successively administered by hypodermic injection of D-gal（1 g.kg-1）.It is made a model of senile mice after 42 d continuation.To measure anti-fatigue performances with the Fatigue Instrument.To measure malondialdehyde（MDA） contents and superoxide dismutase（SOD） activity in brain tissue of senile mice.Result： The n-butanol extract from P.oleracea（0.5 g.kg-1） and the ethanol extract from P.oleracea（1 g.kg-1） could improve anti-fatigue performances.The n-butanol extracts from P.oleracea（0.5,0.25 g.kg-1） could decrease MDA contents and SOD activity in brain tissue.The ethanol extract from P.oleracea（1 g.kg-1） could decrease MDA contents n brain tissue.Conclusion： The n-butanol extract from P.oleracea and the ethanol extract from P.oleracea can improve anti-fatigue performances.Its mechanism maybe related by improve SOD activity and decrease MDA contents.The n-butanol extract from P.oleracea is better than the ethanol extract from P.oleracea.

关 键 词：马齿苋 衰老 抗疲劳 超氧化物歧化酶 丙二醛 

分 类 号：R285.5[医药卫生—中药学]