生川乌配不同比例生半夏对乳鼠心肌细胞的毒性作用  

Toxicity of Raw Rhizoma Pinellia in Combination with Raw Radix Aconiti with Different Ratio on Lactation Rat Myocardial Cell

作　　者：许柳[1] 佟继铭[2] 李平[2] 刘玉玲[2] 张树峰[2] 

机构地区：[1]河北联合大学中医学院,河北唐山063000 [2]承德医学院,河北承德067000

出　　处：《中国实验方剂学杂志》2013年第14期250-255,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河北省教育厅重点项目(ZD2010132)

摘　　要：目的:以半数抑制浓度及细胞搏动频率为指标,评价生川乌与生半夏合用对原代乳鼠心肌细胞的毒性影响及与生半夏比例的关系。方法:分离出生3 d内的SD乳鼠心肌细胞进行原代培养。生川乌组(Ⅰ组),生川乌配生半夏1∶0.25(Ⅱ组),1∶0.5(Ⅲ组),1∶1(Ⅳ组)分别以生川乌终质量浓度0,0.016,0.078,0.235,0.392,0.548,0.705,0.861,1.018 g.mL-1(高效液相色谱法测得乌头碱含量分别为0,1,5,15,25,35,45,55,65 mg.L-1)染毒心肌细胞,分别培养1,2,4,12 h,观察各时间点细胞搏动频率,以MTT法检测培养12 h时细胞存活率,应用概率单位法计算半数抑制浓度(IC50)。结果:①染毒12 h,生川乌组抑制率(CI)显著高于其他配伍组(P<0.01),且随着生半夏比例的提高,抑制率降低。②Ⅰ,Ⅱ,Ⅲ,Ⅳ组的IC50分别为0.446,0.460,0.530,0.575 g.mL-1。③在生川乌终质量浓度0.016～0.078 g.mL-1内,1～2 h细胞搏动频率增高,4～12 h搏动频率降低。在0.235～0.861 g.mL-1内,1 h时细胞搏动频率降低,2 h时搏动频率高于1 h,但低于正常水平,4～12 h频率低于1 h。给药1.018 g.mL-1,细胞立即停搏。结论:生半夏配伍生川乌降低了生川乌的毒性,且有明显的剂量依赖关系。Objective： To evaluate the toxicity effect of raw Radix Aconiti and raw Rhizoma Pinelliae on lactation rat＇s myocardial cells and the relationship between the toxicity effect and different proportion.Method： The SD lactation rat＇s myocardial cells were separated and cultivated when born in 3 days.The four groups were raw Radix Aconite,Radix Aconiti and raw Rhizoma Pinelliae 1∶ 0.25,1∶ 0.5,1 ∶ 1（Ⅰ,Ⅱ,Ⅲ,Ⅳ） were given the final concentration of the Radix Aconiti of 0,0.016,0.078,0.235,0.392,0.548,0.705,0.861,1.018 g.mL-1[aconitine（AC） dose were 0,1,5,15,25,35,45,55,65 mg.L-1]to the myocardiaL cells,respectively cultivating 1,2,4,12 h,to observe the cell pulsation frequency in different time,with MTT method to detect the percentage of the cell survival at 12 hours,and calculate half inhibition concentration（IC50） by probability unit method.Result： ①At 12 h,the inhibition rate（CI） of raw Radix Aconiti group was significantly higher than other groups（P 0.01）,and with the increasing of theproportion of the raw pinellia tuber,the inhibition rate was decreased.② The IC50 ofⅠ,Ⅱ,Ⅲ,Ⅳ group were 0.446,0.460,0.530,0.575 g.mL-1③ When the final concentration of raw Radix Aconiti is 0.016-0.078 g.mL-1,the cell pulsation frequency was increased in 1-2 h,and reduced in 4-12 h.Between 0.235-0.861 g.mL-1,the cell pulsation frequency was reduced in 1 h,and rised when 2 h,but less than normal level,then falled in 4-12 h.Dose with 1.018 g.mL-1,the cells were immediately arrested.Conclusion： The addition of Raw pinellia reduced the toxicity of the raw Radix Aconiti,and there is a significant dose-response relationship.

关 键 词：心肌细胞 生川乌 生半夏 心肌毒性 半数抑制浓度 

分 类 号：R285.5[医药卫生—中药学]