腺苷拮抗大鼠低氧性右心室心肌肥厚的机制  

Antagonism role of adenosine in right ventricular hypertrophy induced by hypoxia in rat

作　　者：王波[2] 谭建新[1] 

机构地区：[1]广东医学院附属医院儿科,广东湛江524001 [2]深圳市第二人民医院儿科

出　　处：《中华实用儿科临床杂志》2013年第13期1017-1019,共3页Chinese Journal of Applied Clinical Pediatrics

基　　金：广东省自然科学基金（8152402301000010）

摘　　要：目的 观察腺苷及其激动剂拮抗低氧性右心室心肌肥厚的作用，并探讨其作用机制。方法 56 只Sprague-Dawley大鼠随机分成常氧对照组、低氧对照组、低氧＋腺苷组、低氧 ＋腺苷 A1受体激动剂（CPA）组、低氧＋腺苷A2受体激动剂（NECA）组、低氧＋CPA ＋腺苷A1受体抑制剂（DPCPX）组、低氧 ＋NECA ＋腺苷 A2b受体抑制剂（MRS1754）组，每组各8只。大鼠置于低氧仓（氧体积分数为95～105 mL/L）连续低氧21 d。各处理组于低氧第7天使用植入式胶囊渗透压泵给药，持续14 d，实验结束时，测定大鼠右心室心肌肥厚程度，采用PCR法检测右心室心肌组织中肌球蛋白重链（β-MHC）mRNA、G蛋白调节因子4（RGS4）mRNA 的表达。结果 1.大鼠慢性持续低氧21 d后，低氧对照组右心室/（左心室＋室间隔）［RV/（LV ＋S）］和右心室/体质量（RV/BW）比值均明显高于常氧对照组（P均 〈0.001）；低氧 ＋腺苷组、低氧 ＋ CPA 组 RV/（LV ＋S）、RV/BW 比值均明显低于低氧对照组（P 均 〈0.05）；低氧 ＋ NECA 组 RV/（LV ＋ S）比值明显低于低氧对照组（P 〈 0.05）。2.低氧对照组β-MHC mRNA表达水平高于常氧对照组（P 〈0.001），低氧对照组 RGS4 mRNA 表达水平低于常氧对照组（P均 〈0.001）；低氧 ＋腺苷组、低氧 ＋CPA 组、低氧 ＋NECA 组 β-MHC mRNA 表达水平均低于低氧对照组（P均〈0.001），低氧＋腺苷组、低氧 ＋CPA 组、低氧 ＋NECA 组 RGS4 mRNA 表达水平均高于低氧对照组（P均〈0.001）。结论 腺苷可通过调控RGS4表达拮抗低氧性右心室心肌肥厚。Objective To investigate the antagonism role of adenosine and its agonists in hypoxia-induced right ventricular hypertrophy in rat. Methods Fifty-six Sprague-Dawley rats were randomly divided into 7 groups,in-cluding normoxia control group, hypoxia control group, hypoxia plus adenosine group, hypoxia plus adenosine A1 recep-tor agonist（CPA） group, hypoxia plus adenosine A2 receptor agonist （NECA） group, hypoxia plus CPA and adenosine A1 receptor antagonist（DPCPX） group,hypoxia plus NECA and adenosine A2b receptor antagonist（MRS1754） group. There were 8 rats in each group. Rats in all groups except normoxia group were exposed to hypoxia for 21 days（ oxygen volume concentration of 95-105 mL/L）. On the 7th day, agents were continuously delivered with micro osmotic pump for 14 days. At the end of the experiment,both right ventricular to left ventricular plus septum weight[ RV/（ LV ＋ S） ] ratio and right ventricular to body weight （RV/BW） ratio were measured, and the expression of myosin heavy chain （ β-MHC） mRNA and regulator of G protein signaling 4 （RGS4） mRNA in the right ventricular myocardial tissues were de-tected by PCR method. Results 1. After chronic hypoxia for 21 days, the ratios of RV/（ LV ＋ S） and RV/BW in the hypoxia control group were significantly higher than those in the normoxia control group respectively（ all P 〈 0.001 ） ; the ratios of RV/（ LV ＋ S） and RV/BW in the hypoxia plus adenosine group and hypoxia plus CPA group were signifi-cantly lower than those in the hypoxia control group（ all P 〈 0.05） ;the ratio of RV/（ LV ＋ S） in the hypoxia plus NE- CA group was significantly lower than that in the hypoxia control group（ P 〈 0. 05 ）. 2. The expression level of β-MHC mRNA in the hypoxia control group was higher than that in the normoxia control group（P 〈 0. 001 ）, and the expression level of RGS4 mRNA in the hypoxia group was lower than that in the normoxia group（P 〈 0.001 ） ;the expression levels of β-MHC mRNA

关 键 词：腺苷 低氧 心肌肥厚 G蛋白调节因子4 

分 类 号：R965[医药卫生—药理学]