耐受性小鼠骨髓源性树突状细胞的培养及生物学功能  

The cultivation of tolerant bone marrow-derived dendritic cells of mice and their biological function in vitro

作　　者：马院院[1] 王春锋[1] 杨木清[1] 丁晶[1] 李济宇[1] 

机构地区：[1]上海市新华医院普外科,200092

出　　处：《实用医学杂志》2013年第14期2265-2267,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:30600598);上海市科学技术委员会医学重点攻关项目(编号:09411952500);上海市科委青年科技启明星资助计划(编号:10QH1401800);上海市教育委员会曙光计划(编号:10SG20);国家自然科学基金(编号:81270555)

摘　　要：目的:建立简便的耐受性小鼠骨髓源性树突状细胞(dendriticcells,DCs)的诱导培养,并探讨耐受性DCs的生物学功能。方法:直接冲出小鼠骨髓源细胞,用IL-4和GM-CSF诱导分化,对终细胞进行形态的观察、表型的鉴定、和功能检测。结果:诱导培养7d的DCs低表达表面共刺激分子为非成熟状态,非成熟DCs也可刺激淋巴细胞增殖并且分泌的IL-10显著升高。结论:这种获得耐受性小鼠骨髓源性DCs的方法相对简便,耐受性DCs可刺激淋巴细胞增殖且高分泌IL-10。Objective To explore a relatively simple method of inductive cultivation of tolerant bone marrowderived dendritic cells（DCs） of mice and to investigate the biological function of tolerant DCs.Methods Bone marrow-derived DCs were induced by GM-CSF and IL-4 from the culture of mice bone marrow cells in vitro.The morphology,phenotype,and function of DCs were observed.Results DCs with low expression of surface costimulate molecular in day 7 were in immature state,these DCs could stimulate the proliferation of lymphocytes and the secretion of IL-10.Conclusion The method used in this study was a simple method to harvest tolerant bone marrow-derived DCs,which could stimulate the proliferation of lymphocytes and the secretion of IL-10.

关 键 词：树突状细胞 耐受性 IL-10 

分 类 号：R392[医药卫生—免疫学]