白蛋白希夫碱配合物结合体清除自由基性能的研究  

Albumin conjugating Schiff-bases metal complexes for removing radical

作　　者：赵婷婷[1] 何玉风[1] 王燕[1] 毛娟娟[1] 王荣民[1] 杨艳丽[1] 

机构地区：[1]生态环境相关高分子材料教育部重点实验室,甘肃省高分子材料重点实验室,西北师范大学化学化工学院,甘肃兰州730070

出　　处：《化学研究与应用》2013年第7期970-975,共6页Chemical Research and Application

基　　金：国家自然科学基金项目(21263024;21244003)资助

摘　　要：以水溶性生物高分子牛血清白蛋白(BSA)为载体,与小分子N-4-羟基水杨醛缩苯丙氨酸希夫碱金属配合物(HOSalpheM)结合,制得水溶性生物高分子金属配合物结合体(BSA-HOSalpheM,M=Co、Mn、Zn、Cu、Fe)。采用FT-IR、UV-Vis、CD和Native-PAGE电泳对其结构进行了表征和分析。采用NBT光化学还原法考察了BSA-HOSalpheM清除羟基自由基(·OH)的活性。结果表明:HOSalpheM具有一定的抗·OH活性,与生物高分子结合后,结合体BSA-HOSalpheM具有良好的水溶性和·OH清除能力。Using bovine serum albumin （BSA）as the water-soluble biopolymer carrier, and N-4-hydroxy-salicylaldehyde-phenylala- nine Schiff base metal complexes （ HOSalPheM, M = Co, M n, Zn, Cu, Fe） as active center, the water-soluble biopolymer metal com- plex（BSA-HOSalPheM） was prepared with conjugation. The structure of BSA-HOSalPheM was determined by FT-IR, UV-Vis, CD and Native-PAGE electrophoresis. The antioxidant radical activity of BSA-HOSalPheM was investigated by NBT photochemical re- duction, and compared with the small molecule amino acid Schiff base （HOSalPheM） and BSA. The mechanism of scavenging hy- droxyl radical were discussed. The results show that amino acid Schiff base has a certain antioxidant activity. After being combined with the biopolymer carrier, the combination （BSA-HOSalPheM） show the excellent water solubility and scavenging ability of hydrox- yl radical.

关 键 词：牛血清白蛋白 水溶性生物高分子结合体 苯丙氨酸希夫碱金属配合物 清除·OH活性 

分 类 号：O636.9[理学—高分子化学]