硝普钠分光光度法测定对乙酰氨基酚  

Spectrophotometric determination of paracetamol by using sodium nitroprusside

作　　者：范秉琳[1,2] 王全坤[1] 李晶[1] 李全民[1] 

机构地区：[1]河南师范大学化学与化工学院,河南新乡453007 [2]新乡医学院基础医学院,河南新乡453003

出　　处：《化学研究与应用》2013年第7期1069-1072,共4页Chemical Research and Application

基　　金：河南省基础与前沿研究计划项目(102300410255;132300410205)资助

摘　　要：建立了硝普钠分光光度法测定对乙酰氨基酚的方法。研究表明:在碱性条件下,对乙酰氨基酚与硝普钠以1∶2的化学计量比反应形成一种绿色产物,其最大吸收波长为698nm。对乙酰氨基酚在1.7～102μg·mL-1浓度范围内与吸光度成良好线性关系,线性回归方程为A=0.02804+0.02285c(μg·mL-1),线性相关系数r=0.9986,表观摩尔吸收系数ε698=3.5×103L·mol-1·cm-1,检测限(3s·k-1)为1.42μg·mL-1。本方法成功应用于药物样品中对乙酰氨基酚含量的测定,回收率在97.6%～102.0%,结果满意。A novel method is established to determine paracetamol by using sodium nitroprussidet. In an alkaline solution a green product is formed by the reaction of paracetamol and sodium nitroprusside. The reaction stoichiometric ratio is 1：2 and the colored product is formed with the maximum wavelength at 698nm. Absorbency is linear with the concentration of paracetamol in the range of 1.7 - 102 μg· mL-1 , and the regression equation is A = 0. 02804 ＋ 0. 02285e （μg· mL-1 ） with a correlation coefficient of 0. 9986. The apparent molar absorption coefficient and the detection limit is 3.5×103 L·mol-1 · cm-1 and 1.42μg· mL-1, respective- ly. The proposed method has been applied to the determination of paracetamol in tablets with the recoveries of 97. 6% - 102.0%.

关 键 词：硝普钠 对乙酰氨基酚 分光光度法 

分 类 号：O657.32[理学—分析化学]