我国玉米上一个水稻黑条矮缩病毒重排体的ORF序列(英文)  

Com plete ORF Sequences of a Natural Rice black-streaked dwarf virus Reassortant from M aize in China

作　　者：阴筱[1] 许斐斐[1] 朱芹芹[1] 刘志强[1] 张广民[1] 李向东[1] 

机构地区：[1]山东农业大学植物保护学院植物病毒研究室,泰安271018

出　　处：《植物病理学报》2013年第4期354-361,共8页Acta Phytopathologica Sinica

基　　金：Modern maize industrial system of Shandong province

摘　　要：由水稻黑条矮缩病毒(Rice black-streaked dwarf virus,RBSDV)引起的粗缩病是我国玉米生产中的一种毁灭性病害。本文报道从山东枣庄玉米上得到的RBSDV分离物SDZZ10所有可读框(ORF)的序列。与全基因组序列已知的2个RBS-DV分离物Hbm和Zjr比较,SDZZ10的大多数ORF与Hbm相应ORF的核苷酸序列一致率更高,其蛋白与Hbm相应蛋白的氨基酸一致率也更高,但SDZZ10的ORF3,ORF4,ORF9-2和ORF10与Zjr相应ORF的核苷酸一致率更高,P4,P9-1和P9-2与Zjr相应蛋白的氨基酸一致率更高。根据ORF8和ORF10构建的系统进化树中,SDZZ10分别属于不同的组,说明SDZZ10是一个自然发生的重排体。Maize rough dwarf disease caused by rice black-streaked dwarf virus （RBSDV） is a destructive disease in China. Here, we reported the complete genomic sequences of all the open reading frames （ORFs） in SDZZ10, a RBSDV isolate from maize in Zaozhuang, Shandong Province of China. Comparing with two RBSDV isolates whose complete genornic sequences were available, the most ORFs and corresponding proteins of SDZZ10 shared higher nucleotide （nt） or amino acid （aa） identities with RBSDV-Hbm; While ORFs 3, 4, 9-2 and 10 of SDZZ10 shared higher nt identities with RBSDV-Zjr, P4, P9-1 and P9-2 shared higher aa identities with RBSDV-Zjr. Phylogenetic analyses of ORF8 and ORF10 showed that SDZZ10 was clustered in- to different groups, indicating that SDZZ10 is a natural reassortant.

关 键 词：水稻黑条矮缩病毒 开放读框 重排体 玉米粗缩病 

分 类 号：S432.41[农业科学—植物病理学]