番茄细菌性软腐病病原的分离与鉴定  

Isolation and identification of the pathogen causing tomato bacterial soft rot

作　　者：张国庆[1] 翟婧[1] 周青[1] 王笑 崔舟琦[1] 谢关林[1] 

机构地区：[1]浙江大学生物技术研究所水稻生物学国家重点实验室,农业部病虫分子生物学重点开放实验室,杭州310029 [2]宁波市江北区农技推广服务总站,宁波315021

出　　处：《植物病理学报》2013年第4期435-439,共5页Acta Phytopathologica Sinica

基　　金：农业部公益性行业(农业)科研专项(201003029;201003066);国家自然科学基金资助项目(30871655)

摘　　要：Pectobacterium carotovorum subsp．carotovorum（syn．Erwinia carotovora subsp．carotovora）是引起作物腐烂的常见病原细菌，主要侵染亚热带及温带地区的作物。如，大白菜、马铃薯、茄子、辣椒等。Tomato bacterial soft rot has been become seriously in recent years in some areas of China. How- ever, little is known about the causal agent. It was isolated and identified for effective management of the disease. The symptoms of the disease were described and the pathogen was tested in clony morphology, patho- genicity, transmission electron microscopy, biolog and fat acid analysis. The results indicated that the causal agent was belonged to Pectobacterium. The 16S rDNA was also sequenced and had 99% similarity with Pec- tobacterium carotovorum subsp, carotovorum. It was formed in a group with P. c. subsp, carotovorum ATCC15713, LMG2404, DSM30168 and Pectobacterium carotovorum subsp, odoriferum LMG17566, which indicated that the pathogen was Pectobacterium carotovorum.

关 键 词：病原细菌 细菌性软腐病 鉴定 分离 番茄 温带地区 亚热带 大白菜 

分 类 号：S432.42[农业科学—植物病理学]