棉籽蛋白源ACE抑制肽的制备过程中脱盐技术的研究  

Study on Desalination in Preparation Process of ACE-Inhibitor Peptide from Cottonseed Protein

作　　者：刘军[1] 徐志宏[1] 魏振承[1] 廖森泰[1] 穆利霞[1] 

机构地区：[1]广东省农业科学院蚕业与农产品加工研究所,广东省农产品加工重点实验室,广州510610

出　　处：《中国粮油学报》2013年第7期36-40,共5页Journal of the Chinese Cereals and Oils Association

基　　金：国家科技支撑计划(2012BAD33B10/2012BAD37B08);广东省科技项目(2011A080403019/2011B010500022)

摘　　要：综合比较了001×7、201×7离子交换树脂、MWCO100透析袋和DA201-C大孔吸附树脂对棉籽蛋白水解液的脱盐效果,分析了DA201-C树脂脱盐对棉籽蛋白水解液ACE抑制活性和氨基酸组成的影响,并对Sephadex G-25分离纯化获得ACE抑制肽各组分进行活性测定。结果表明:DA201-C大孔吸附树脂的脱盐效果最好,控制流速为3 mL/min时,脱盐率可达到94.78%,氮回收率为86.32%,脱盐后疏水性氨基酸得到了有效富集,ACE抑制率显著提高。分离纯化后的ACE抑制肽各组分中,组分Ⅲ的抑制率最高,分子质量小于1 000 u。Cottonseed hydrolysates were desalted by 001 ×7,201 ×7 iron exchange resin, MWC0100 dialytic bags and DA201 - C macroporous adsorption resin, their desalination effects were compared synthetically. The effect of ACE inhibitor activity and amino acid composition were analyzed after desalination by DA201 -C resin, and the ACE inhibitor activity of several compositions after isolation with Sephadex G -25 was determined. The results showed that when the velocity of flow was controlled at 3 mL/min, DA201 - C macroporous adsorption resin had the best desalination effect;desalination ratio reached to 94.78% ;nitrogen recovery ratio was 86.32%. Hydrophobic amino acid was enriched and ACE inhibitor activity was improved effectively after desalination. Among several compositions after isola- tion and purification ,the composition Ⅲ had the best ACE -Inhibitor activity with molecular weight less than 1 000 u.

关 键 词：棉籽蛋白 纯化 脱盐 ACE抑制肽 

分 类 号：TS2[轻工技术与工程—食品科学与工程]