单双侧电针治疗对CCI模型大鼠脊髓SP基因表达影响的比较  

Comparison of Effects of Single and Bilateral Sides Electro-acupuncture on SP Gene in Spinal Cord of CCI Model Rats

作　　者：王金金[1] 屠文展[2] 蒋松鹤[2] 余清[3] 楼新法[3] 

机构地区：[1]温州医学院附属第一医院,浙江温州325000 [2]温州医学院附属第二医院,浙江温州325027 [3]温州医学院,浙江温州325035

出　　处：《中华中医药学刊》2013年第7期1624-1626,I0010,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省教育厅资助项目(20051157);温州市科技局科研基金资助项目(Y20080031)

摘　　要：目的:比较单双侧电针对坐骨神经慢性挤压伤(CCI)大鼠脊髓中P物质(SP)基因表达的影响。方法:35只雄性SD大鼠造模后随机分为假模组(J组)、对照组(A组)、患侧电针组(B组)、健侧电针组(C组)、双侧电针组(D组)。各电针组治疗9 d后,观察电针前后痛阈变化;逆转录聚合酶链式反应(RT-PCR)技术法检测脊髓背角中SP基因的表达。结果:B、C、D各电针治疗组之间比较显示:SP基因和β-actin光密度比值D组<B组<C组,彼此间均具有差异(P<0.05)。大鼠脊髓背角中SP基因表达与针刺前后的痛阈差值具有显著相关性(P<0.01)。结论:电针疗法分别应用于大鼠患侧、健侧、双侧足三里和阳陵泉穴,均可通过抑制大鼠脊髓背角中SP基因的表达,产生镇痛作用。Objective：To compare the expression changes of Substance P gene in spinal cord of chronic constriction injury of the sciatic nerve model rats by sick side,healthy side and bilateral sides electro-acupuncture treatment.Methods：After CCI model was successfully made,35 male Sprague-Dawley rats were randomized into false group（Group J）,control group（Group A）,sick side electro-acupuncture group（Group B,）,healthy side electro-acupuncture group（Group C）,bilateral sides electro-acupuncture group（Group D）.Each electro-acupuncture group was treated for 9 days,then the changes of pain threshold before and after the electro-acupuncture were observed;and the expression of SP gene in spinal dorsal horn was detected by using reverse transcriptase-polymerase chain reaction.Result：Compared among Group B,C and D：the value of Z/R Reinheit Zahl of SP gene and β-actin was：Group DGroup BGroup C,there was difference between each other（P〈0.05）.Analyzing correlation between SP gene and difference value of the pain threshold：sp gene expression in rats spinal dorsal horn and difference value of the pain threshold had obvious statistic correlationship（P〈0.01）.Conclusion：Electro-acupuncture applied separately in ST36 and GB 34 on sick side,healthy side and bilateral sides,generated the analgesia function by suppressing SP gene expression in rats spinal dorsal horn.

关 键 词：电针 SP CCI模型 镇痛 

分 类 号：R246[医药卫生—针灸推拿学]