筋骨草总黄酮对MsPGN大鼠血清IL-1、TNF-α水平的影响  

Experimental Effects Study of Total Flavonoids of Ajuga on Interleukin-1 and Tumor Necrosis Factor-α in Mesangial Proliferative Glomerulonephritis Rats

作　　者：彭卫华[1,2,3] 南丽红[4] 贾铷[4] 方泰惠[5] 吴符火[4] 徐曾涛[4] 

机构地区：[1]南京军区福州总医院肾脏科 [2]厦门大学附属东方医院 [3]福建医科大学福总临床医学院,福建福州350025 [4]福建中医药大学药学院,福建福州350108 [5]南京中医药大学药学院,江苏南京210029

出　　处：《中华中医药学刊》2013年第7期1627-1630,I0011,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：福建省卫生厅中医药科研重点课题资助项目(wzzsb0906);陈可冀中西医结合发展基金.福建省中西医结合老年性疾病重点实验室开放课题资助项目(CKJ2008088);福建中医药大学校管课题资助项目(X2009069)

摘　　要：目的:探讨筋骨草总黄酮对系膜增生性肾小球肾炎(MsPGN)大鼠的干预作用机制。方法:采用改良慢性血清病MsPGN大鼠模型,于造模第5w末检测尿蛋白,将尿蛋白阳性者随机分为模型组、雷公藤多甙(TWP)组、不同剂量筋骨草总黄酮(TFA)组,另设正常对照组。于造模第6w起开始给药,6w后,测定各组大鼠的24 h尿蛋白定量、血生化和肾组织病理变化,放射免疫分析法观察TFA对MsPGN模型大鼠血清中白细胞介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)的影响。结果:TFA可显著降低尿蛋白,明显减小系膜区面积占毛细血管丛面积的百分比、减轻肾脏损害(P<0.05或P<0.01);高、中剂量的TFA治疗效果与TWP相近(P>0.05)。模型对照组大鼠血清中IL-1(ng.mL-1)、TNF-α(ng.mL-1)的表达明显高于正常对照组(IL-1 0.829±0.251 vs0.238±0.081,P<0.01;TNF-α8.43±2.50 vs 1.52±0.55,P<0.01);干预性治疗后,TFA高、中剂量组、TWP组大鼠血清中IL-1、TNF-α的表达较模型对照组明显降低(IL-1 0.497±0.106,0.543±0.116,0.474±0.076,P<0.01;TNF-α5.52±1.74,5.86±0.92,5.01±1.78,P<0.01);TFA高、中剂量组IL-1、TNF-α表达虽略高于TWP组,但无统计学差异(P>0.05)。结论:筋骨草总黄酮对MsPGN大鼠有较好的治疗作用,降低IL-1和TNF-α的水平,可能是其治疗作用机制之一。Objective：To investigate the effects of total flavonoids of Ajuga（TFA） in mesangial proliferative glomerulonephritis（MsPGN） rats and its mechanism.Methods：MsPGN rat was induced by the improved chronic serum sickness model after five weeks.The rats with positive urinary protein rats were randomly divided into model group,triptergium wilfordii polyglycosidium（TWP） group and TFA group with different dosage,normal rats wasere considered as the control group.MsPGN rats were treated with drugs since the sixth week;six weeks after succussfully modelingeeding six weeks later,all rats were detected 24 h urinary protein,and blood biochemistry,pathomorphological change in renal tissue,serum Interleukin-1（IL-1） and tumor necrosis factor-α（TNF-α） wasere measured by radioimmunoassay.Results：Compared with model group,TFA can significantly reduce the urinary protein,decrease BUN,Scr,TG,Tch,mitigate the morphological damage,and significantly increase TP（P〈0.05 or P〈0.01）.The effects of high-dosage（2.160 g kg-1） TFA and middle-dosage（1.080 g/kg-1） TFA were as similar as those of TWP（P〈0.05）.The serum levels of IL-1 and TNF-α（ng·mL-1） in model control wasere significantly higher than that in normal control group;.With the intervention trherapy of high-dosage TFA,middle-dosage TFA and TWP,the expressions of serum IL-1 and TNF-α wasere significantly decreased compared with model control group（.The expressions of IL-1 and TNF-α in high-dosage TFA,and middle-dosage TFA groups wasere slightly higher than those in TWP group,with no significant differences（P〈0.05）.Conclusion：Total flavonoids of Ajuga has a therapeutic effect on MsPGN rats;Rreducing the expressions of serum IL-1 and TNF-α,which may be one of the mechanisms of MsPGN treatment.

关 键 词：筋骨草 总黄酮 系膜增生性肾小球肾炎 白细胞介素-1 肿瘤坏死因子-α 

分 类 号：R285.5[医药卫生—中药学]