鸡眼草的生药学研究  

Pharmacognosy Research on Herba Kummerowiae

作　　者：刘豪华[1] 胡春萍 许莉 程韵菲[1] 靳鑫[1] 祝峥[1] 崔泽实[1] 张东方[1] 

机构地区：[1]中国医科大学药学院生药学教研室,辽宁沈阳110001 [2]鞍山生物工程有限公司,辽宁鞍山114000 [3]辽宁省中药研究所,辽宁沈阳110041

出　　处：《中华中医药学刊》2013年第7期1637-1639,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省科学事业研究公益基金资助项目(2012006005);沈阳市科学技术计划项目(F11-241-00;F12-264-4-01)

摘　　要：目的:建立民间用药鸡眼草的质量标准评价方法。方法:采用性状鉴别和叶、茎横切面及粉末的显微鉴别方法鉴别鸡眼草,采用高效液相色谱法测定其中的山柰酚,色谱柱:C18(4.6 mm×150 mm,5μm);流动相:甲醇-0.1%磷酸(63∶37);检测波长:370 nm。结果:在叶、茎横切面显微图可见乳汁管和韧皮纤维束。在粉末显微图中,可见非腺毛、晶纤维束等。采用HPLC法测定鸡眼草中山柰酚的含量,稳定性良好,精密度良好,重复性良好,加样回收率良好,每1 g鸡眼草中山柰酚含量为0.046～0.049 mg。结论:鸡眼草的药材性状、显微鉴别特征明显,对该生药鉴别具有重要意义。HPLC法可用于测定鸡眼草中山柰酚的含量。Objective： To set up a method of quality control of Herba Kummerowiae.Methods： Character identificationand microscopical identification of leaf,stem and powder were used.Content of kaempferol from Herba Kummerowiae was determined by HPLC and chromatographic column was C 18（4.6 mm × 150 mm,5 μm）;mobile phase was methanol 0.1% phosphonic acid（63 ∶ 37）;wave length was 370 nm.Results： Laticiferous tube and phloem fiber were discovered from leaf and stem.Nonglandular hair and crystalfibrin were found from powder.Stability,precision,repetition of HPLC was better.Content of kaempferol was 0.046 ～ 0.049 mg/g in Herba Kummerowiae.Conclusion： Character identification and microscopical identification of leaf,stem and powder of Herba Kummerowiae are distinct.The content determination of kaempferol from Herba Kummerowiae is feasible.

关 键 词：鸡眼草 显微鉴别 山柰酚 含量测定 

分 类 号：R284.1[医药卫生—中药学]