枳壳炮制前后HPLC特征图谱及橙皮苷含量测定研究  

Study on HPLC specific Chromatograms and Content of Hesperidin of Crude and Processed Fructus Aurantii

作　　者：陈学建[1] 秦昆明[2,3] 李鹏 蔡宝昌[2,3] 

机构地区：[1]连云港市赣榆县中医院,江苏连云港222100 [2]南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210029 [3]南京海昌中药集团有限公司,江苏南京210061

出　　处：《中华中医药学刊》2013年第7期1671-1674,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：南京市科技计划资助项目(201007005);南京市公共技术服务平台建设资助项目(201105007);江苏省企业博士集聚计划资助项目

摘　　要：目的:建立枳壳生品和炮制品的HPLC特征图谱分析方法,同时测定炮制前后橙皮苷的含量。方法:采用Kromasil C 18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%甲酸水溶液梯度洗脱法,检测波长254 nm,流速1.0 mL.min-1,柱温35℃。结果:3个产地的枳壳特征图谱有一定差异;炮制后橙皮苷的含量略有降低。结论:采用特征图谱结合指标性成分含量测定的方法可以较好的控制药材及饮片的质量。该法精密度高、重现性好,所测组分均达到基线分离,可以用于炮制前后枳壳的质量评价。Objective： To cpmpare the HPLC specific chromatograms and content of hesperidin between crude and processed Fructus Aurantii.Methods： The HPLC separation was performed on a Kromasil C18 column（250 mm× 4.6 mm,5 μm） by gradient elution with a mixture of acetonitrile-0.2% aqueous formic acid as a mobile phase,the flow rate was 1.0 mL·min^-1,and the column temperature was 35 ℃.The detection wavelength was set at 254 nm.Results：Specific chromatograms of Fructus Aurantii from three main producing areas showed higer similarity.The content of hesperidin both decreased a little in processed Fructus Aurantii.Conclusions： Specific chromatograms combined with marker compounds determination can be used for quality control of Chinese medical materials and Chinese herbal pieces.This method shows high precision,good repeatability and contents are separation.It can be used for assessing the quality of crude and processed Fructus Aurantii.

关 键 词：枳壳 炮制 特征图谱 橙皮苷 

分 类 号：R284.1[医药卫生—中药学]