谷氨酸酶促反应中光替代谷氨酸氧化酶的研究  

A Study on Light as a Substitute for Glutaminic Acid Oxidase in the Enzymatic Reaction of Glutaminic Acid

作　　者：王细生[1] 王志红[1] 郑翔鹏 蔡维平[1] 许金钩[1] 

机构地区：[1]厦门大学化学系,现代分析科学教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2000年第5期637-641,共5页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金资助项目!(B 96 0 0 3)

摘　　要：在弱碱性 (p H=10 .6 )介质中 ,紫外光的照射引起谷氨酸的光化学反应 ,产生 H2 O2 和 NH3 ,与谷氨酸氧化酶存在下的酶促反应等效 .以萘斯勒试剂使产物中的 NH3 显色并用分光光度法测定 ,以及以氯化血红素 (hemin)作为过氧化物酶的替代物 ,催化产物中的 H2 O2 氧化、偶联对羟基苯乙酸(HPA)的荧光反应并进行荧光检测 .据此分别建立了间接测定谷氨酸的新方法 .In week alkaline medium(pH=10.6),photochemical reaction of glutaminic acid can occur under the irradiation of UV light and produces NH 3 and H 2O 2.This reaction is equal in value to enzymatic reaction of glutaminic acid in the presence of glutaminic acid oxidase.NH 3 produced by photochemical reaction is coloured by Nessler′s reagent and the absorbance is measured.However, using hemin as a substitute of peroxidase,H 2O 2 produced by photochemical reaction oxidazes p hydroxyphenyl acetic acid(HPA) and couses the coupled fluorescence reaction.Accodingly,the spectrophotometry and fluorimetry have been developed for indirect determination of glutaminic acid,respectively.

关 键 词：光化学反应 测定 谷氨酸 光替代酶 谷氨酸氧化酶 

分 类 号：Q517.03[生物学—生物化学] O657.3[理学—分析化学]