不同尺寸、形貌金纳米粒子小鼠体内急性毒性研究  

Acute toxicity studies of gold nanoparticles of different size and morphology in mice

作　　者：李媛[1,2] 王小慧[2] 庄远[1] 张华欣[1] 阎少多[2] 詹林盛[2] 

机构地区：[1]解放军总医院,北京100853 [2]军事医学科学院野战输血研究所,北京100850

出　　处：《军事医学》2013年第6期450-454,共5页Military Medical Sciences

摘　　要：目的考察不同尺寸、不同形貌的金纳米粒子的急性毒性。方法采用聚乙二醇(polyethylene glycol,PEG)对系列不同尺度、不同形貌的金纳米粒子进行修饰,通过测定zeta电位及表面等离子共振谱对修饰后纳米粒子的电性质及在生理盐水中的稳定性进行考察,最后将4种修饰后的金纳米材料通过腹腔注射入小鼠体内并在24 h后进行血常规及急性时相反应蛋白检查。结果 PEG修饰后的金纳米颗粒能够在生理盐水环境中稳定存在。血常规结果表明,50 nm粒径的棒状金纳米粒子会引起白细胞数量的明显下降,50 nm粒径的球形金纳米粒子则引起血小板数量的减少,而粒径为10 nm及90 nm的球形金纳米粒子组血常规指标无明显变化。而且,4种纳米粒子均不会引起小鼠体内急性时相反应蛋白的明显升高。结论对于金纳米粒子来说,产生急性毒性与否似乎与其尺度之间并不存在所谓的线性依赖关系,而50 nm可能是生物体产生急性毒性的敏感尺度。Objective To evaluate the acute toxicity of gold nanoparticles（GNPs） of different size and morphology.Methods First,gold nanoparticles of different size and morphology were modified by polyethylene glycol（PEG）.The as-prepared gold nanoparticles were characterized by zeta potential measurements and surface plasmon resonance to study their stability in physiological saline.Then,the mice were respectively given one-time intraperitoneal administration with these four types of modified gold nanoparticles.After 24 hours,the mice were sacrificed and the blood was collected for routine blood examination and acute phase protein analysis.Results PEG-modified gold nanoparticles were stable in the physiological environment.The results showed that the rod-shaped gold nanoparticles of about 50 nm caused the decrease in the number of white blood cells while the sphere-shaped gold nanoparticles of c.a.50 nm could reduce the number of platelets significantly.The other two kinds of gold nanoparticles,namely GNPs of the size of 10 nm and 90 nm,caused no significant changes in the blood examination.Furthermore,none the four types of gold nanoparticles elevated the expression level of acute phase proteins of serum amyloid A（SAA） and C-reactive protein（CRP）.Conclusion For gold nanoparticles,there seems to be no so-called linear dependence between the scale and acute toxicity,however,the scale of 50 nm may be the sensitive scale to induce acute toxicity.

关 键 词：金纳米粒子 血液学试验 聚乙烯二醇类 

分 类 号：R99[医药卫生—毒理学] TB383.1[医药卫生—药学]