全自动血培养系统与双相血培养系统应用对比  

BDBACTEC FX and biphasic blood culture system

作　　者：王凤莲[1] 陈海 李欢 麦珍 黎元莉 

机构地区：[1]海南省干部疗养院检验科,海南海口571100 [2]三亚市人民医院检验科,海南三亚572000

出　　处：《中华医院感染学杂志》2013年第14期3555-3557,共3页Chinese Journal of Nosocomiology

基　　金：三亚市重点实验室项目(L1216)

摘　　要：目的比较及评价全自动血培养系统BDBACTEC FX与双相血培养系统的临床应用,以提高检测率。方法回顾性分析奥普双相血培养系统检测1140份血标本和全自动血培养系统BDBACTEC FX检测1338份血标本,对检出阳性的病原菌种类、时间和阳性率、污染率进行比较分析。结果双相血培养检测标本1140份,检出病原菌70份,阳性率为6.1%;BDBACTEC FX检测标本1338份,检出病原菌128份,阳性率为9.6%;双相血培养系统最快阳性检出时间为20h,BDBACTEC FX最快阳性检出时间为3h30min,24、48、72h内检出的阳性数分别占78.0%、90.8%、98.9%。结论应用BDBACTEC FX全自动血培养系统最大优点缩短了阳性检出时间,为抢救患者赢得了时间,其次检出细菌种类增多,提高了血培养的阳性率,但成本大;奥普双相血的特点为也能较快、较准地检出病原菌,具有成本较低、使用方便的特点,适于县级医院使用。OBJECTIVE To compare and evaluate the clinical application of automated blood culture system（BDBACTEC FX）and the two-phase blood culture system so as to improve the detection rate.METHODS A total of 1140 blood specimens detected with opp biphasic blood culture system and 1338 blood samples detected with BDBACTEC FX were retrospectively,then the bacterial species detected positive,time,positive rate,and contamination rate were compared and analyzed.RESULTS Of 1140 blood specimens detected with opp biphasic blood culture system,totally 70 strains of pathogens were isolated with the positive rate of 6.1%;of 1338 blood samples detected with BDBACTEC FX,totally 128 strains of pathogens were isolated with the positive rate of 9.6%.The fastest-positive detection time of the biphasic blood culture system was 20h,the BDBACTEC FX 3 hours and 5half,and the strains detected positive within 24 hours,48 hours,or 72 hours accounted for 78.0%,90.8%,and 98.9%,respectively.CONCLUSIONBDBACTEC FX has the greatest advantage of shortening the positive detection time and gaining time to save the patient,followed by detecting varied species of pathogens,and improving the positive rate of blood culture,however,it is of large cost;the Aopu biphasic blood culture system can also detect the pathogens in a rapid and accurate manner,it is of low cost and is suitable for the country hospitals to use.

关 键 词：血培养 病原菌 检出时间 BDBACTEC FX 双相血培养 

分 类 号：R446[医药卫生—诊断学]