异丙酚联合贝那普利预处理在大鼠心肌缺血再灌注损伤中的抗氧化作用  

The antioxidant protective effect of the combination of propofol and benazepril preconditioning against myocardial ischemia reperfusion injury in rats

作　　者：李建辉[1] 侯晓敏[2] 于丁[3] 李军霞[4] 

机构地区：[1]邢台市人民医院麻醉科,河北邢台054000 [2]河北医科大学研究生学院,河北石家庄050017 [3]河北医科大学第二医院心外科,河北石家庄0500001 [4]河北医科大学药理教研室,河北石家庄050017

出　　处：《中国医院药学杂志》2013年第13期1049-1053,共5页Chinese Journal of Hospital Pharmacy

摘　　要：目的:建立大鼠缺血再灌注模型,观察异丙酚和贝那普利联合预处理对大鼠心肌缺血再灌注损伤的抗氧化保护作用。方法:采用结扎大鼠冠状动脉左前降支法制备心肌缺血再灌注模型。大鼠随机分成5组:假手术组;缺血再灌注模型组;异丙酚组;贝那普利组;异丙酚和贝那普利联合预处理组。用试剂盒检测血清中乳酸脱氢酶(LDH)、肌酸磷酸激酶(CK)、肌酸磷酸激酶同工酶(CK-MB)含量、丙二醛(MDA)浓度及超氧化物歧化酶(S0D),以及心肌组织MDA浓度及S0D、谷胱甘肽过氧化物酶(GSH-Px)活性。结果:异丙酚和贝那普利联合用药可进一步明显降低血清中LDH、CK、CK-MB活性和MDA含量;升高血清中SOD活性,升高心肌组织SOD和GSH-Px活性,降低心肌组织MDA含量。结论:异丙酚和贝那普利对大鼠心肌缺血再灌注损伤有协同的保护作用。OBJECTIVE To investigate the antioxidant protective effect of the combination of propofol and benazepril preconditioning against myocardial ischemia reperfusion injury in rats.METHODS SD rats were randomly divided into five groups： sham group;myocardial ischmia/reperfusion group;propofol group;benazepril group;combination of propofol and benazepril preconditioning group.The following indexes were detected by using kits： the levels of CK,CK-MB,LDH,SOD,MDA in serum and the activity of SOD,MDA,and GSH-Px in myocardium tissue were detected using kits.RESULTS Propofol and benazepril decreased the activity of CK,CK-MB,LDH and the content of MDA in serum,increased the activity of SOD in serum,increased the activity of SOD,GSH-Px and decreased the level of MDA in myocardium tissue.CONCLUSION Propofol and benazepril in combination have synergetic effect on proctecting cardiomyocyte from ischemia/reperfusion injury.

关 键 词：异丙酚 贝那普利 联合预处理 心肌缺血再灌注 

分 类 号：R972.6[医药卫生—药品]