HPLC法测定苦豆子总碱结肠定位释放片中生物碱  

Determination of alkaloids in Sophora alopecuroides Colon-specific Release Tablets by HPLC

作　　者：刘振龙[1,2] 张丽[2] 魏蕾初[2] 郑堰心[2] 邓虹珠[2] 

机构地区：[1]广东省食品药品检验所,广东广州510180 [2]南方医科大学,广东广州510515

出　　处：《中成药》2013年第7期1453-1456,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立苦豆子总碱结肠定位释放片中生物碱的测定方法。方法采用HPLC法,乙腈-0.05 mol/L磷酸二氢钾(三乙胺调pH值大于6.45),梯度洗脱,柱温35℃,检测波长205 nm,体积流量1.0 mL/min。结果野靛碱、苦豆碱、槐定碱、氧化苦参碱、氧化槐果碱、槐果碱、苦参碱、莱曼碱8种生物碱分离良好,线性范围分别为0.044 8～0.896 0μg、0.113 0～2.260 0μg、1.536 6～30.732 0μg、0.322 2～6.444 0μg、0.108 4～2.168 0μg、0.404 8～8.096 0μg、0.033 2～0.664 0μg、0.036 2～0.724 0μg,加样回收率大于96%,供试品溶液在24 h内稳定性良好,8种生物碱总量的转移率大于90%。结论该方法简便,检出率高,可以作为苦豆子总碱结肠定位释放片的测定方法。AIM To establish a method for determining alkaloids in Sophora alopecuroides Colon-specific Release Tablets.METHODS The HPLC experiment was performed with the elute of acetonitrile-0.05 mol/L potassium dihydrogen phosphate（triethylamine adjust pH value greater than 6.45） in gradient elution mode with the column temperature of 35 ℃,detection wavelength of 205 nm,and flow rate of 1.0 mL/min.RESULTS Eight kinds of alkaloids were separated well and linear ranges were 0.044 8-0.896 0 μg,0.113 0-2.260 0 μg,1.536 6-30.732 0 μg,0.322 2-6.444 0 μg,0.108 4-2.168 0 μg,0.404 8-8.096 0 μg,0.033 2-0.664 0 μg and 0.036 2-0.724 0 μg,respectively.All recovery rates were above 96%.The sample solution in 24 h was stable.The transfer rate of eight alkaloids was over 90%.CONCLUSION The method is simple and can be used to determine alkaloids in Sophora alopecuroides Colon-specific Release Tablets.

关 键 词：苦豆子总碱 结肠定位释放片 野靛碱 苦豆碱 槐定碱 氧化苦参碱 氧化槐果碱 槐果碱 苦参碱 莱曼碱 

分 类 号：R927.2[医药卫生—药学]