桃根癌病菌拮抗放线菌抑菌物质的分离纯化与结构鉴定  

Identification of antimicrobial composition isolated and purified from antagonistic actinomycetes against Agrobacterium tumefaciens of peach

作　　者：侯柄竹[1] 王树芳[1] 马焕普[1] 刘志民[1] 

机构地区：[1]北京农学院植物科学技术学院北京102206

出　　处：《微生物学通报》2013年第7期1186-1192,共7页Microbiology China

基　　金：北京市自然科学基金项目(No.5112010);北京市教委资助项目(No.KM200910020001)

摘　　要：【目的】为明确根癌拮抗放线菌G-19的抑菌活性物质。【方法】采用Sephadex LH-20柱层析、高效液相色谱及中压制备色谱等技术,对桃根癌拮抗放线菌G-19发酵液中的抑菌活性成分进行分离纯化,并通过LC-MS、NMR对其结构进行鉴定。【结果】分离得到化合物G-19-I的结构为邻苯二甲酸二丁酯(DBP),化合物G-19-II的结构为2-(4-羟基苯基)-3,4-二氢-2H-苯并吡喃-3,4,5,7-四醇,俗称无色天竺葵素。【结论】阐明了放线菌G-19抑菌活性成分的物质基础。[Objective] To isolate and identify the antimicrobial active component extracted from fermentation broth of antagonistic actinomycetes strain G-19 against peach crown gall （Agrobacterium tumefaciens）. [Methods] Sephadex LH-20 chromatography, HPLC and mid-die-pressure chromatography were used for isolation and purification. LC-MS, NMR were ap- plied to identify structure of the active component. [Results] Compound G-19-I was isolated and structurally identified as 1,2-benzenedicarboxylic acid, 1,2-dibutyl ester （DBP）; compound G-19-II was isolated and structurally identified as 2-（4-hydroxyphenyl）-3,4-dihydro- 2H-chromene-3,4,5,7-tetrol, commonly known as leucopelargonidin. ]Conclusion] The mate- rial basis of the antibacterial constituents in antagonistic actinomycetes strain G-19 was further clarified

关 键 词：桃树根癌病 放线菌 邻苯二甲酸二丁酯 无色天竺葵素 

分 类 号：S436.629[农业科学—农业昆虫与害虫防治]