16SrDNA序列分析在鉴定布鲁氏菌中的应用  

Application of the 16S rDNA sequence analysis method in identification of Brucella

作　　者：汤旭[1,2] 姜海[2] 赵鸿雁[2] 朴冬日[2] 田国忠[2] 张秋香[3] 崔步云[2] 王桂琴[1] 

机构地区：[1]山西医科大学微生物与免疫教研室,山西太原030001 [2]传染病预防控制国家重点实验室中国疾病预防控制中心传染病预防控制所布鲁氏菌病室,北京102206 [3]山西省疾病预防控制中心,山西太原030012

出　　处：《微生物学通报》2013年第7期1290-1296,共7页Microbiology China

基　　金：国家自然科学基金项目(No.81271900);国家科技重大专项项目(No.2011ZX10004-001);国家973计划项目(No.2010CB530201)

摘　　要：【目的】建立布鲁氏菌的16S rDNA序列分析方法,评价该方法鉴定布鲁氏菌的特异性和实用性。【方法】用PCR扩增布鲁氏菌的16S rDNA片段,将扩增的产物纯化后测序,从GenBank下载与布鲁氏菌易发生血清学交叉反应的细菌的16S rDNA序列。使用DNAMAN软件进16S rDNA序列相似性分析。【结果】在布鲁氏菌中16S rDNA核苷酸序列相似性达到了99.74%,而与其他有血清型交叉反应的菌株相比较,16S rDNA序列间有显著差异。【结论】16S rDNA序列分析是一种快速、简便、特异的鉴定布鲁氏菌的方法之一。[Objective] To establish the 16S rDNA sequence analysis method to identifiy Brucella and evaluate the specificity and feasibility of this method. [Methods] 16S rDNA were amplified from Brucella by polymerase chain reaction （PCR） method and the purified product were directly sequenced for further analysis. The 16S rDNA sequence of the bacteria that are known to cross-react serologically with Brucella were downloaded from the GenBank. DNAMAN was used for comparison of 16S rDNA sequence. [Results] Brucella 16S rDNA sequence similarity reached 99.74%, Brucella 16S rDNA sequence to serologically related bacteria had more pronounced differences. [Conclusion] 16S rDNA sequence analyses is a rapid, simple diagnostic and specific method.

关 键 词：布鲁氏菌 16S RDNA 鉴定 

分 类 号：R516.7[医药卫生—内科学]