禽致病性大肠杆菌江苏、安徽分离株的生物学特性分析  

Characterization of Avian Pathogenic Escherichia coli isolated from Jiangsu and Anhui provinces

作　　者：白灏[1,2] 冀辉[1] 韩先干[2] 龚建森[3] 董洪亮[2] 丁铲[2] 祁克宗[1] 于圣青[2] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]中国农业科学院上海兽医研究所,上海200241 [3]中国农业科学院家禽研究所,江苏扬州225125

出　　处：《微生物学通报》2013年第7期1315-1322,共8页Microbiology China

基　　金：国家自然科学基金项目(No.31001078,30871851)

摘　　要：【目的】研究禽致病性大肠杆菌(Avian Pathogenic Escherichia coli,APEC)江苏、安徽分离株的优势血清型,并分析其生物学特性。【方法】对分离自病禽的细菌进行鉴定,采用玻片凝集法测定禽致病性大肠杆菌的血清型,PCR方法检测14种毒力基因的分布,采用美国临床和实验室标准化研究所的方法进行药物敏感性检测,改良结晶紫半定量法检测分离细菌的生物被膜形成能力。【结果】共分离到禽致病性大肠杆菌56株,血清型检测结果表明,O78血清型占64.29%,为主要血清型。毒力基因检测显示,fimC、pfs、ompA和luxS的阳性率超过90%。药物敏感性检测显示,58.93%的菌株对8种以上的药物耐受。生物被膜检测显示,有16株细菌生物被膜形成能力为中等以上,其中68.75%的菌株耐8种以上的药物。【结论】O78为主要流行的血清型。fimC、pfs、ompA和luxS基因为APEC保守基因。多重耐药性仍很普遍,细菌生物被膜与耐药性具有相关性。[Objective] The aim of this study was to study the main serotypes and the biologi- cal characteristics of Avian Pathogenic Escherichia coli （APEC） isolated from Jiangsu and Anhui provinces. [Methods] The tested strains were isolated from sick poultry and identified by slide agglutination to determine serotypes. Virulence-associated genes were identified by PCR and the drug susceptibilities were detected according to Clinical and Laboratory Stan- dards Institute （CLSI）. Moreover, the biofilm-forming abilities were detected through im- proved crystal violet semi-quantitative method. [Results] In total 56 strains were isolated and identified. Slide agglutination results show that 078 was the main serotype accounted for 64.29% of the isolates. PCR amplification results of the virulence-associated genes showed thatfimC, pfs, ompA and luxS were amplified from more than 90% of the tested strains. Drug sensitivity test results showed that 58.93% of the strains were resistant to more than 8 antibi- otics. Biofilm formation assay showed that 16 isolates produced moderate or strong biofilm in vitro, in which 68.75% were resistant to more than 8 antibiotics. [Conclusion] 078 was the main serotype among the isolates. The genes offimC, pfs, ompA and luxS were conserved in APEC. Multi-antibiotic resistance was very common and drug resistance seemed relative to biofilm-forming ability.

关 键 词：禽致病性大肠杆菌 血清型 毒力基因 耐药性 生物被膜 

分 类 号：S852.61[农业科学—基础兽医学]