LC-MS/MS法测定人血浆中地芬尼多的含量及其在案件中的应用  

LC-MS/MS determination of difenidol in human blood and application in law cases

作　　者：栾玉静[1] 董颖[1] 王瑞花[1] 侯小平[1] 王芳琳[1] 于忠山[1] 

机构地区：[1]公安部物证鉴定中心,北京100038

出　　处：《药物分析杂志》2013年第7期1137-1140,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：公安部应用创新计划(2010YYCXGAES098)

摘　　要：目的:利用高效液相色谱-质谱联用仪建立人血浆中地芬尼多的定性定量方法。方法:应用OasisTMHLB小柱进行固相萃取法提取,XBridge RP-18色谱柱分离。结果:在该条件下,人血浆中地芬尼多的线性范围为1.0～100.0 ng.mL-1,最小检出限为0.5 ng.mL-1。地芬尼多在1.0～100.0 ng.mL-1浓度范围内的回收率均在80%以上。结论:本研究所建立的方法适用于血浆中地芬尼多的定性定量检验。Objective： To develop a qualitative and quantitative method by LC-MS /MS for the determination of difenidol in human blood.Methods： Difenidol was extracted from serum by OasisTM cartridges and separated on the XBridge RP-18 column.Results： Under this condition,the calibration curve of difenidol in human plasma was linear within the injection amount ranging from 1.0 ng.mL-1 to 100 ng.mL-1,the minimum limit of detection was 0.5 ng.mL-1 and the recovery was above 80% for difenidol within the range of 1.0-100 ng.mL-1.Conclusion： The described LC-MS /MS method can be applied to the qualitative and quantitative determination of difenidol in human plasma.

关 键 词：高效液相色谱-质谱联用 法医毒物分析 地芬尼多 固相萃取 定性定量分析 

分 类 号：R917[医药卫生—药物分析学]