UPLC法同时测定蒙药壮西六味散中胡椒碱和荜茇宁的含量  

UPLC simultaneous determination of piperine and piperlonguminine in the Mongolian medicine Zhuangxi-6

作　　者：宝贵荣[1,2] 领小[2] 孟和[2] 博.格日勒图[2] 

机构地区：[1]天津大学药物科学与技术学院,天津300072 [2]内蒙古大学高分子化学及蒙药研究所,呼和浩特010021

出　　处：《药物分析杂志》2013年第7期1223-1226,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(编号:81060366)资助

摘　　要：目的:建立了超高液相色谱法同时测定蒙药壮西六味散中胡椒碱和荜茇宁的含量。方法:色谱分离采用ACQUITY UP-LC BEH C18(100 mm×2.1 mm,1.7μm)色谱柱,以乙腈-水(50∶50,v/v)为流动相,流速0.2 mL.min-1,检测波长338 nm,柱温40℃,进样量0.5μL。结果:胡椒碱和荜茇宁分别在0.1～100μg·mL-1(r=0.9993)和0.02～25μg·mL-1(r=0.9997)浓度范围内具有良好的线性关系,其加标回收率(n=6)分别为97.3%～99.3%和95.2%～97.2%,RSD分别为0.91%～2.4%和1.0%～3.3%。结论:该方法快速、准确,适用于同时测定药物中胡椒碱和荜茇宁含量,可为壮西六味散的质量控制提供一定的参考。Objective： To develop an UPLC method for the simultaneous determination of piperine and piperlonguminine in Mongolian medicine Zhuangxi-6.Methods： Chromatographic separations were accomplished on an ACQUITY UPLC BEH C18 column（100 mm × 2.1 mm,1.7 μm） at 40 ℃.The mobile phase consisted of acetonitrile and water in a 50∶ 50（v / v） mixture with a flow rate of 0.2 mL.min-1,and the injection volume was 0.5 μL.The detection was performed at the wavelength of 338 nm.Results： The calibration curves showed good linearity of piperine and piperlonguminine in the concentration range from 0.1-100 μg.mL-1（r =0.9993） and 0.02-25 μg.mL-1（r = 0.9997）.The recoveries of piperine and piperlonguminine were 97.3%-99.3% and 95.25% 97.2%,and RSDs were 0.91-2.4% and 0.1%-3.3%,respectively.Conclusion： The established method is rapid and accurate,which is suitable for the simultaneous determination of piperine and piperlonguminine in Mongolian medicine,and can be used for the quality control of Zhuangxi-6.

关 键 词：壮西六味散 胡椒碱 荜茇宁 超高效液相色谱 蒙药质量控制 

分 类 号：R917[医药卫生—药物分析学]