RP-HPLC法测定提宗龙胆和线叶龙胆部位中不同齐墩果酸和熊果酸含量  

Determination of oleanolic acid and ursolic acid in different parts of Gentiana tizuensis and Gentiana farreri by RP-HPLC

作　　者：黄浩[1,2] 邹盛勤[1,3] 

机构地区：[1]宜春学院化学与生物工程学院,宜春336000 [2]江西省高校应用化学与化学生物学重点实验室,宜春336000 [3]江西省天然药物活性成分研究重点实验室,宜春336000

出　　处：《药物分析杂志》2013年第7期1239-1242,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家"863"计划重点项目(2002AA2Z3217)资助;江西省教育厅科技项目(赣教技[2008]GJJ08530号)

摘　　要：目的:建立反相高效液相色谱法同时测定提宗龙胆和线叶龙胆不同部位中齐墩果酸和熊果酸的含量。方法:采用Kro-masil C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.2%磷酸水溶液(90∶10)为流动相,流速0.8 mL·min-1,检测波长205nm,柱温30℃。结果:齐墩果酸和熊果酸均达到基线分离,线性范围分别为0.652～6.52μg(r=0.9998)和0.996～9.96μg(r=0.9997);平均回收率(n=6)分别为101.2%(RSD=1.6%)和98.0%(RSD=1.7%)。结论:采用RP-HPLC法同时测定提宗龙胆和线叶龙胆中齐墩果酸和熊果酸的含量,方法简便、快速、准确,可用于提宗龙胆和线叶龙胆的质量控制。Objective： To develop a RP-HPLC method for simultaneous determination of oleanolic acid and ursolic acid in different parts of Gentiana tizuensis and Gentiana farreri.Method： The samples were isolated on a Kromasil C18column（4.6 mm × 250 mm,5 μm） with methanol-water containing 0.2 % phosphoric acid（90∶ 10） at a flow rate of 0.8 mL.min-1.The detection wavelength was 205 nm and the column temperature was controlled at 30 ℃.Results： Oleanolic acid and ursolic acid were base-isolated,and the linear ranges were 0.652-6.52 μg（r = 0.9998） and 0.996-9.96 μg（r = 0.9997）,with the average recoveries（n = 6） at 101.2%（RSD = 1.6%） and 98.0%（RSD =1.7%）,respectively.Conclusion： The established RP-HPLC method can simultaneously determine the content of oleanolic acid and ursolic acid in Gentiana tizuensis and Gentiana farreri.The method is convenient,rapid and reliable,which can be applied to the quality control of Gentiana tizuensis and Gentiana farreri.

关 键 词：提宗龙胆 线叶龙胆 齐墩果酸 熊果酸 高效液相色谱法 峰纯度分析 中药质量控制 

分 类 号：R917[医药卫生—药物分析学]