榄仁树叶提取物(LR-98)对实验性肝损伤的防护作用  

PROTECTIVE EFFECTS OF LR-98 ON HEPATOTOXICITY INDUCED BY CARBON TETRACHLORIDE AND D-GALACTOSAMINE IN MICE

作　　者：徐力致[1] 高静[1] 朱俐[2] 徐曼 卢是月 赵晓宁[1] 张祖暄[1] 

机构地区：[1]南京大学医学院,江苏南京210093 [2]南通医学院,江苏南通226001 [3]南京军医学院,江苏南京210099

出　　处：《南京大学学报（自然科学版）》2000年第2期197-201,共5页Journal of Nanjing University（Natural Science）

基　　金：江苏省自然科学基金!(No :BK990 5 1)资助项目

摘　　要：采用生化检测并结合形态学观察 ,研究了榄仁叶提取物 (LR - 98)对小鼠实验性化学肝损伤的防护作用 .发现在急性肝损伤实验中 ,LR - 98能对抗四氯化碳 (CCl4 )引起的肝细胞核膜皱缩、周边异染色质增加、核固缩及髓鞘样变化等亚微结构的病理学改变 .LR - 98不仅显著剂量依赖性抑制CCl4 所致小鼠血清谷丙转氨酶 (sGPT)活性的升高 ,完全阻抑CCl4 所致小鼠肝组织中超氧化物歧化酶 (SOD)活性的下降 ,且可完全逆转D -氨基半乳糖 (D -GalN)诱发的sGPT活性的升高 .在慢性肝损伤 (肝硬化 )实验中 ,高剂量LR 98可完全逆转由CCl4 所致sGPT活性、肝羟脯氨酸含量的显著增高 ,以及SOD活性的下降 .结果表明 ,LR - 98对急慢性肝损伤有良好的防护作用 ,其机理可能与抗脂质过氧化有关 .In the present paper, seurm glutamic pyruvic transaminase (sGPT), superoxide dismatase (SOD) and liver hydroxyproline were determined to investigate the inhibitory effects of LR-98(extract from Terminalia catappa L. ) on carbon tetrachloride (CCl 4) or D GalN induced acute or chronic hepatic injury in mice. Ultrastructural Morphological changes were also observed under transmission electron microscope (TEM). In acute hepatic injury trial, ①it was found that LR-98 could prevent CCl 4 induced nuclear membrane shrinkage, condensed heterochromatin increase, karyopyknosis and medullary sheath like structure occurrence. ②sGPT activity was significantly increased ( P <0.01), and liver SOD activity was significantly reduced ( P < 0.01 ) after intraperitoneal injection of 1% CCl 4. With oral pretreatment of LR-98 for 1 week, changes in sGPT could be blocked ( P <0.01) in a dose dependent manner. Furthermore, changes in SOD activity could be completly inhibited by all different doses of LR-98 ( P <0.01). ③LR-98 could also reverse the increase in sGPT activity induced by intraperitoneal treatment of D-GalN(800?mg/kg). In chronic liver damages (experimental hepatocirrhosis) induced by CCl 4, both sGPT activity and liver hydroxyproline content were significantly increased ( P <0.01), while SOD activity was significantly reduced compared to control values ( P <0.01) . With the oral pretreatment of LR-98, all the above changes induced by CCl 4 could be partly (lower dose) or completely (higher dose)blocked. But the treatment of LR-98(500?mg/kg) alone didn't affect any parameters detected. The above data indicate that LR-98 has hepatoprotective activity. The mechanisms underlying its protective effects may be related to antioxidant activity.

关 键 词：榄仁树叶提取物 实验性 防护作用 肝损伤 LR-98 

分 类 号：R259.751[医药卫生—中西医结合] R287.55[医药卫生—中医内科学]