几种氯代芳烃对鱼体内性激素水平的影响研究  

EFFECTS OF SEVERAL CHLOROAROMATIC HYDROCARBONS ON PLASMA SEX STEROIDS IN CARASSIUS AURATUS

作　　者：杨兴烨[1] 尹大强[1] 孙爱龙[2] 翟卫中[3] 

机构地区：[1]污染控制与资源化研究国家重点实验室南京大学环境科学与工程系,江苏南京210093 [2]南京大学生物化学系,江苏南京210093 [3]南京铁道医学院附属医院核医学科,江苏南京210009

出　　处：《南京大学学报（自然科学版）》2000年第2期219-223,共5页Journal of Nanjing University（Natural Science）

基　　金：国家自然科学基金!资助项目 (No :39770 15 3)

摘　　要：选取了工业废水中普遍存在的几种氯代芳烃 ,以腹腔注射的方法对鲫鱼进行染毒 .分别用放射免疫测定方法测定未染毒和染毒的鲫鱼体内的性激素 (睾酮和 17β -雌二醇 )含量 ,并对测定结果进行比较 .结果发现染毒鲫鱼体内睾酮的浓度水平与未染毒鲫鱼体内睾酮水平相比存在着明显的差异 ,而 17β -雌二醇浓度水平与未染毒鲫鱼体内 17β -雌二醇水平相比基本上没有显著差异 .表明上述氯代芳烃类化合物进入水生动物尤其是高等水生动物体内后 ,能显著影响睾酮的合成与释放及其正常生理功能 ,而对 17βDuring the recent decade there has been increasing concern at the levels of industrial and agricultural contaminants in the aquatic environment and the possible effect of such pollution on both human health and the well being of animal populations.Since some of these contaminants which are called endocrine disrupters have oestrogenic activities and can mimic the role of oestrogen and disturb the reproductive function of animals and human beings,more and more efforts have been made to elucidate the potential effects of these contaminants of animals especially aquatic animals.In this study,several chloroaromatic hydrocarbons chlorobenzene,1,3 dichlorobenzene,1,4 dichlorobenzene and p chloro methylbenzene,which are generally presented in industrial effluents,were selected to be studied under laboratory conditions for their effects of plasma sex steroids (testosterone and 17β estradiol)in carp (Carassius auratus) to determine whether these chemicals may act as endocrine disrupters and cause threats to reproduction of aquatic species.The 4 chloroaromatic hydrocarbons were injected into carp at different doses separately,and after 2 weeks,carp plasma was collected,centrifuged and extracted by dichloromethane,and sex steroids levels were detected using radioimmunology assay (RIA).The results showed that in comparison with the mean concentration of testosterone which was (4?072.582±227.44) pg/mL in control groups,the concentrations of testosterone in chemical treated groups significantly increased ( P < 0.05 ),and in contrast with the effect on testosterone levels,nearly no significant differences exist between concentrations of 17β estradiol in chemical treated fish and the relative 17β estradiol level of (190.66±20.16)pg/mL in control groups ( P >0.05).These results suggested that although these 4 chloroaromatic hydrocarbons may have no obvious effects on the 17β estradiol level in aquatic animals such as fish,they contribute to the disturbance of testosterone levels in fish and may cause gr

关 键 词：氯代芳烃 鲫鱼 雌二醇 睾酮 性激素 鱼体 废水 

分 类 号：X520.332.5[环境科学与工程—环境工程]