PEG和NaCl胁迫对菘蓝不同四倍体株系靛蓝、靛玉红含量的影响  

Effects of PEG and NaCl Stress on the Contents of Indigotin and Indirubin in Isatis indigotica Tetraploids

作　　者：客绍英[1] 马艳芝[1] 

机构地区：[1]唐山师范学院生命科学系,河北唐山063000

出　　处：《中药材》2013年第4期525-527,共3页Journal of Chinese Medicinal Materials

基　　金：国家科技部子课题(2011BAI07B05-4);河北省科技厅项目(10965511D);唐山市科技重大项目(12130203A)

摘　　要：目的:为选育抗旱耐盐菘蓝优良四倍体株系提供参考。方法:以生长30 d的菘蓝二倍体(对照组)和人工选育四倍体株系组培幼苗为材料。分别采用2 g/L的NaCl溶液50 mL,15%的PEG-6000溶液50 mL浸泡12 h后将溶液倒出,再培养7 d。HPLC测定靛蓝、靛玉红含量。结果:胁迫后,不同四倍体株系靛蓝、靛玉红含量变化不尽相同,PEG胁迫后供试材料DB3、DB5、DB12在提高有效成分上表现均优于其他材料,NaCl胁迫后DB2、DB3、DB12则在提高有效成分含量上表现优异。结论:DB3和DB12可作为重点株系进行下一步研究。Objective：To provide a reference evidence for choosing salt-resistent and drought-resistent varieties of Isatis indigotica Fort.. Methods ： The tissue culture seedlings of different Isatis indigotica tetraploids were used as plant materials. The diploid was used as CK,which were cultured for 30 days. 2 g/L NaCl and 15% PEG-6000 were used as stress treatment respectively for 12 hours,then poured out the solution, the materials were continuously cultured for 7 days. The contents of indirubin and indigotin were determined by HPLC. Results ：The contents of indigotin and indirubin of DB3, DB5 and DB12 with PEG treatment and DB2, DB3 and DB12 with NaCl treatment were increased. Conelusion：DB3 and DB12 can be further studied as reproduction materials.

关 键 词：菘蓝 NaCl PEG 胁迫 靛蓝 靛玉红 

分 类 号：R282.6[医药卫生—中药学]